Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지

Promotion of in vitro growth and rooting of micropropagated shoots in Spathiphyllum floribundum by the addition of liquid medium

액체배지 첨가에 의한 Spathiphyllum floribundum 신초의 기내생육 및 발근 촉진

  • Published : 2001.07.01
Download PDF
⟨ Previous Next ⟩

Abstract

This experiments were carrid out to examine the effects of liquid medium addition in same vessels on shoot elongation and rooting, and soil survival of plantlets after the shoot cluster sections of Spathiphyllum floribundum 'Cupid' were pre-cultured. The shoot clusters with 3 to 4 small shoots were proliferated on LS medium supplemented with 2.0 mg/L BA for 8 weeks, and then 15 mL of various kinds of liquid medium was added in the same vessels. The addition of 15 mL liquid medium containing l/2 MS macro elements, 50 g/L sucrose and 5.0∼10.0 g/L activated charcoal was significantly stimulated the elongation and rooting of proliferated shoots. The medium addition was resulted in the enhanced soil survival of plantlets.

Spathiphyllum을 기내에서 증식시킨 후, 액체배지를 첨가하여 신초의 생장 및 발근을 촉진시키고, 기외에서 순화율을 향상시켜 생산비를 감소시키기 위하여 일련의 실험을 실시하였다. 3∼4개의 신초를 가지고 있는 Spathiphyllum floribundum 'Cupid' 의 신초 cluster 절편체를 BA 2.0 mg/L가 첨가된 LS배지에서 8주간 증식시킨 후에 15 mL의 액체배지를 동일용기에 첨가하였다. 액체배지 첨가는 1/2 MS 다량요소+sucrose 50 g/L +활성탄 5.0∼10.0 g/L가 첨가된 배지 15 mL를 첨가하는 것이 식물체의 기내생장 및 발근에 양호하였으며, 온실에서 perlite와 vermiculite가 1 : 1로 혼합된 용토에 발근된 식물체를 재식하면 95% 이상 생존하였다.

Keywords

References

  1. Planta v.115 Hapliod plants from anthers of tobacco-enhancement with charcoal Anagnostakis, S. L
  2. Sci Hort v.14 A scheme for commercial propagation of ornamental plants by tissue culture Debergh, P. C;Maene, L. J
  3. Physiol Plant v.65 Carbon dioxide and ethylene evolution in the culture atmosphere of Magnolia sultured in vitro De Proft, M. P;Maene, M. J;Devergh, P. C
  4. Proc Fla State Hort Sci v.91 Establishment of tissue culture grown plants in the green house environment Doman, A;Davidson, S;Willians, C
  5. Physiol Plant v.34 Effects of activated charcoal on growth and morphogensis in cell cultures Fridborg, G;Eriksson, T
  6. Physiol Plant v.43 The effect of activated charcoal on tissu cultures: absorption of metabolites inhibiting morphogenesis Fridborg, G;Pedersen, M;Landstrom, L-E;Eriksson, T
  7. Physiol Plant v.18 Organic growth factor requirments of tabacco tissue culture Linsmaire, E. M;Skoog, F
  8. Plant Cell Tiss Organ Cult v.5 Liquid medium addition to established tissue cultures to improve elongation and rooting in vitro Maene, L;Debergh, P
  9. Acta Hort v.131 Rooting of tissue cultured plants under in vivo conditions Maene, L. J;Debergh, P. C
  10. Physiol. Plant v.15 A revised medium for rapid growth and biossays with tobacco tissue culture Murashige, T;Skoog, F
  11. Plant Growth Regul v.25 Effect of different environmental conditions in vitro on sucrose metabolism and antiozidant enzymatic activities in cultured shoots of Nicotiana tabacum L. Piqueras, A;Han. B. H;Van Huylenbroeck, J. M;Debergh,P. C
  12. Z Pflanzenphysiol v.89 Some effects of activated charcoal as an additive to plant to plant tissue culture media Weatherhead, M. A;Burdon, J;Henshaw, G. G
  13. Environ & Ezp Bot v.21 Hardening carnation plants regenerated from shoot tips cultured in vitro Ziv, M;Meir. G;Halevy, A