지치 (Lithospermum erythrorhizon S.) Callus 생장에 미치는 저선량 $\gamma$선의 효과

Effects of Low Dose $\gamma$ Radiation on Callus Growth of Lithospermum erythrorhizon S.

  • 황혜연 (충남대학교 원예학과) ;
  • 김재성 (한국원자력연구소 동위원소·방사선 응용연구팀) ;
  • 이영복 (충남대학교 원예학과)
  • 발행 : 2001.11.01

초록

본 실험은 지치 (Lithospermum erythrorhizon)의 뿌리조직으로부터 유도한 캘러스에 ${\gamma}$선을 조사하여 배양기간과 배지조성 및 명.암배양의 조건에 따라 ${\gamma}$선 조사가 캘러스 생장촉진 효과가 있는지를 검토하고자 수행하였다. 지치의 캘러스 생장은 전체적으로 암배양에서보다 명배양에서 효과적이었다. r선 조사에 의한 효과에 있어서는 LLB2N2배지의 8 Gy에서 캘러스의 생장에 효과가 가장 좋았고, LLK2I0.2배지에서는 16 Gy에서 캘러스 생장효과가 좋았지만 암배양에서의 ${\gamma}$선처리 효과는 없었다. 명배양에서 LS배지에 BA 1 mg/L와 NAA 1 mg/L를 첨가할 경우, BA 2mg/L와 NAA 2 mg/L를 첨가할 때보다 캘러스의 생장효과가 있었으나, 암배양에서는 차이가 없었다. 특정선량에 대한 BA와 NAA의 농도에 있어서는 각각 2 mg/L가 효과적인 것으로 나타났다. MK2I0.2배지는 0.5 Gy와 1 Gy에서 캘러스의 생장에 효과적이었으나, 기본배지의 종류에 있어서는 MS배지보다 LS배지에서 더 효과적이었다. M-9배지에서는 명배양에서도 캘러스의 생장은 저조하였으나, 16 Gy 및 30 Gy에서 캘러스의 생장이 30%이상 증가하였다. 암배양에서는 ${\gamma}$선 조사에 의한 캘러스의 생장효과는 거의 없었다.

The effect of low dose ${\gamma}$-radiation on the callus growth of Lithospermum erythrorhizon S. cultured on different medium and lighting condition was investigated. The 8 Gy irradiation stimulated callus growth on LS medium supplemented with BA 2 mg/L and NAA 2 mg/L, however, the growth of callus was more effective on LS medium supplemented with BA 1 mg/L and NAA 1 mg/L under 16 hrs day light. And on the LS medium containing IAA 0.2 mg/L, 16 Gy irradiation increased the callus growth by supplement with kinetin 2 mg/1 and the effect of kinetin was higher than BA at same concentration. The growth of callus was more vigorous on LS medium than MS medium in general. On M-9 medium, the growth of callus was poor regardless lighting conditions, however, by ${\gamma}$-ray irradiation of 16 Gy or 30 Gy, callus growth rates were increased by 30% or more than 30%, especially, under 16 hrs day light condition.

키워드

참고문헌

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