Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
권호 표지

Effect of Sterols on Phytophthora infestans and Oospore Production on detached Potato Plants

감자 역병균에 대한 스테롤류의 영향 및 감자절편에서의 난포자 형성

  • 이왕휴 (전북대학교 생물자원과학부 농업과학기술연구소) ;
  • 이용훈 (농촌진흥청 호남농업시험장) ;
  • 이두구 (농촌진흥청 호남농업시험장)
  • Published : 2001.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

The effects of media, cholesterol, $\beta$-sitosterol and lecithin on the growth and oospore production of the isolates KM10, U6, CDB6, MHB6, JD1 (A$^2$type) of Phytophthora infestans isolated in Korea and F8l7, DNC303 (A$^1$type), IB908, DN107 (A$^2$type) obtained from Japan were investigated. Mycelium of P. infestans grew better on V-8 juice agar and rye meal agar than on the other media. Oospores were produced most abundantly on V-8 juice agar. Mycelium extended more 16.6, 8.3, and 5.2% on V-8 juice agar supplemented with 5 $\mu\textrm{g}$/$m\ell$ of cholesterol, $\beta$-sitosterol and lecithin, respectively, and oospores are produced 76.0, 58.0, and 34.6 % on V-8juice agar supplemented with 5 $\mu\textrm{g}$/$m\ell$ of cholesterol, $\beta$-sitosterol and lecithin, respectively. Oospores more produced on detached potato plant disks when $A^1$ and $A^2$ type exist simultaneously which indicating that variation of population can occur in the field, but the rate of oospore formation and the number of oospores produced was low and small quantity.

역병균 (Phytophthora infestans)의 균사 신장 및 난포자 형성에 미치는 생리 화학적 영향을 알아보고자 국내에서 분리된 $A^2$형인 KM10, U6, MHB6, CDB6, JD1과 일본에서 분양 받은 $A^1$형인 F8l7, DNC303, $A^2$ 형인 IB908, DN107 균주를 이용하여 공시 배지별 및 V-8A 배지에 cholesterol, $\beta$-sitosterol및 lecithin을 첨가했을 때의 영향을 조사하고, 식물체상에서의 난포자 형성여부를 조사한 결과, 다음과 같은 결과를 얻을 수 있었다. 1. 균사 신장은 V-8A, RMA에서 양호하였고, 난포자 형성은 V-8A에서 가장 많았다. V-8A에 cholesterol, $\beta$-sitosterol 및 lecithin을 첨가하였을 때 접종 10일 후 균사 신장은 무처리 대비 각각 16.6, 8.3, 5.2% 더 신장하였으며, 난포자 형성수는 각각 76.0, 58.0, 34.6% 크게 증가하였다. 2. $A^1$형과 $A^2$형이 동시에 존재할 경우 식물체상에서 난포자를 형성하는지 알아보고자 식물체 조직편에 $A^1$, $A^2$형을 대치 배양하여 난포자 형성을 유도한 결과, 난포자의 형성률 및 수는 적었으나, 식물체 상에서도 난포자 형성이 가능한 것을 확인 할 수 있었는데, 금후 이에 대한 세심한 주의가 필요할 것으로 생각된다.

Keywords

References

  1. Phytopathology v.78 Induction of oospore germination of Phytophthora parasitica Ann, P.J.;Ko, W.H.
  2. Phytopathology v.80 Resistance to fungicide and antibiotics in Phytophthora parasitica: Genetic nature and use in hydrid determination Chang, T.T.;Ko, W.H.
  3. Phytopathology v.73 Occurrence of metalaxyl resistance isolates of Phytophthora infestans in potaot fields in Israel Cohe, Y.;Reuvein, M.
  4. Phytopathology v.83 Phenotypic chages in populations of Phytophthora infestans from Eastern Germany Dagget, S.S.;Gotz, E.;Therrien. C.D.
  5. Neth. J. Pl. Path. v.89 Resisitance to metalaxy in Phytophthora infestans in the Netherlands Davidse, L.C.;Danial, D.L.;Van Weste, C.J.
  6. de Bary in Ireland, Potato Res. v.24 Metalaxyl-resistance strains of Phytophthora infestans(Mont.) Dowley, L.J.;O'sullivan, E.
  7. Ann. Rev. of Phytopathol. v.30 Population genetics and intercontinental migrations of Phytophthora infestans Fry, W.E.;Goodwin, S.B.;Matuszak, J.M.;Spielman, L.J.;Milgroom M.G.
  8. Phytopathology v.50 The nature of sexuality in Phytophthora infestans Phytophthora infestans Galindo, J.A.;Gallegly, M.E.
  9. Phytopathology v.48 Mating types and oospores of Phytophthora infestans in nature in Mexico Galindo, J.A.;Gallegly, M.E.
  10. Plant Pathology v.33 Studies on metalaxyl-resistant Phytophthora infestans in potato crops in south-west Scotland Holmes, S.J.I.;Iselin, K.
  11. Genet, Res. Camb. v.23 The inheritance of drug resistance and compatibility type in Phytophthora drechsleri Khaki, I.A.;Shaw, D.S.
  12. Ann. Rev. Phytopathol. v.26 Hormanal heterothallism and homothallism in Phytophthora Ko, W.H.
  13. Ann. Rev. of Phytopath. Soc. Jpn. v.49 Reassessment of apparent sterol requirement for sexual reproduction in Phytophthora Ko, W.H.;Ho, W.C.
  14. 한식병지 v.10 감자역병균(Phytophthora infestans)의 약제저항성 및 교배형 이왕휴;소만서;최인영
  15. Ann. Phytopathol. Soc. Jpn. v.55 Occurrence of the A² mating type Phytophthora infestans on potato in Japan Masa, A.A.;Kato, M.;Sato, N.;Kobayashi, K.;Ogoshi, A.
  16. Ann. Phytopath. Soc. Jpn. v.57 Formation of oospore by Phytophthora infestans in inoculated potato tissues Mosa, A.A.;Kato, M.;Sato, N.;Kobayashi, K.;Ogoshi, A.
  17. Phytopathology v.65 Comparative effect of monochromatic radiation on the germination of oospore of three Phytophthora spp Ribeiro, O.K.;Zentmyer, G.A.;Erwin, D.C.
  18. Phytopathology v.59 Variation in Pathogenicity among single-oospore cultures of Phytophthora infestans Romero, S.;Erwin, D.C.
  19. Ann. Phytopath. Soc. Jpn. v.65 Distribution of Phytophthora infestans populations in seven Asian countries Nishimura, R.;Sato, K.;Lee, W.H.;Singh, U.P.;Chang, T-.T.;Suryaningsih, E.;Suwonakenee, S.;Lumyong, P.;Chamswarng, C.;Tang, W-.H.;Shrestha, S.K.;Kato, M.;Fujii, N.;Akino, S.;Kondo, N.; kobayashi, K.;Ogoshi, A.
  20. Plant Pathol. v.37 Studies on the inheritance of resistance to metalaxyl in Phytophthora infestans Shattock, R.C.
  21. Plant Pathol. v.39 Phenotypes of Phytophthora infestans collected in England and Wales from 1985 to 1988: mating type, response to metalaxyl and isozyme analysis Shattock, R.C.;Shaw, D.S.;Fyfe, A.M.;Dunn, J.R.;Loney, K.H.;Shattock, J.A.
  22. Plant Pathol. v.34 Occurrence of the rare A² mating type of Phytophthora infestans on imported Egyptian potatoes and the production of sexual progeny with A' mating types from the UK Shaw, D.S.;Hibberd, P.G.;Fyfe, A.M.;Abdel-Sattar, M.A.
  23. Phytopathology v.48 Production and germination oh Phytophthora infestans Smoot, J.J.;Gough, F.J.;Lamey, H.A.;Eichenmuller, J.J.;Gallegly, M.E.
  24. Phytopathology v.60 Development and production of Phytophthora Zentmyer, G.A.;Erwin, D.C.