Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Evaluation of Irritating Potential of Newly Developed Toothpaste in the Hamster Oral Mucous Membrane

  • Kim, Bae-Hwan (Skin Research Institute, Pacific Co. R&D Center) ;
  • Kim, Jin-Woo (Skin Research Institute, Pacific Co. R&D Center) ;
  • Chang, Ih-Seop (Skin Research Institute, Pacific Co. R&D Center) ;
  • Sim, Young-Chul (Skin Research Institute, Pacific Co. R&D Center) ;
  • Lee, Yong-Soon (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
  • Published : 2001.10.01
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Abstract

Oral mucous membrane test using Syrian hamsters was performed to evaluate the reliability as a model system for the assessment of the potentially irritating substances intended for the mucous membranes, and to determine the irritating potential of a new emulsion-type formulated toothpaste. After test substances were implanted into the cheek pouches of hamsters with diluents (20 mg/kg) under pento-barbital sodium anesthesia, we made the comparison in irritation between emulsion-type and dispersion-type of triclosan (TCS) formulations in the range of 0.2% to 0.3%. The emulsion-type formulations using non-ionic surfactant showed less mucosal lesion than other commercial toothpastes with 0.3% TCS, or dispersion-type ones. However, no significant difference in irritation was detected between 0.2% and 0.3% TCS. We report that this hamster cheek pouch method could be a reliable approach for the evaluation slight difference in the irritating potentials of cosmetics and hygiene products intended for the lips or other mucous membranes, and this method showed that the new emulsion-type formulation significantly lowered the TCS-induced toxicity, compared with other commercial toothpastes.

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References

  1. Oral Pathol. Med. v.26 no.4 Triclosan inhibits sodium lauryl sulphate-induced changes in expression of cytokeratin gense in hanster cheek pouch epithelium Baert, J. H.;Veys, R. J.
  2. Oral Surgery v.48 The induction of hyperkeratotic white lesions in hanster cheek pouches with mouthwash Bernstein, M. L.;Carlish, R.
  3. Am. J. Infect. Control v.24 no.3 Triclosan: applications and safety Bhatgava, H. N.;Leonard, P. A.
  4. Laboratory Animal Science v.45 Interlaboratory study for the assessment of potential irritative properties of hygiene products on the hamster cheek pouch Bourrinet, P.;Conduzorgues, J. -P.;Dutertre, H.(et al.)
  5. Appraisal of the safety of Chemicals in Foods, Drugs and Cosmetics Dermal Toxicity Draize, J. H.
  6. Ann. N.Y. Acad. Sci. v.153 Current status of FDA toxicity requirements Fancher, D. E.;Calandra, J. C.
  7. Laboratory Animals v.24 Histomorphology of the hamster cheek pouch Ghoshal, N. G.;Bal, H. S.
  8. Am. J. Infect. Control v.28 no.2 Triclosan: a review of effectiveness and safety in health care settings. Jones, R. D.;Jampani, H. B.;Newman, J. L.;Lee, A. S.
  9. J. Periodontal Res. v.5 Effect of local applications of chothexidine on oral mucosa of the hamster Lindhe, J.;Heyden, G;Svanberg, G.(et al.)
  10. J. Dent. Res. v.65 no.11 Establishment of and improved implantation technique for hamster mucous membrane irritation testing Roy, M.;Whishe, H. I.
  11. Int. J. Exp. Path. v.75 Histological changes in the hamster cheek pouch epithelium induced by topical application of sodium laury sulphate Vey, R. J.;Baert, J. H.;De Boever, J. A.
  12. Eur. J. Oral. Sci. v.106 no.2 Triclosan: cytotoxicity, mode of action, and induction of apoptosis in human gingival cells in vitro Zuckerbraun, H. L.;Babich, H.;May, R.;Sinensky, M. C.