한국연초학회지 (Journal of the Korean Society of Tobacco Science)

한국연초학회 (The Korean Society of Tobacco Science)
권호 표지

돼지 콜레라 바이러스 E2 유전자의 클로닝 및 염기서열분석

Cloning and Sequence Analysis of Hog Cholera Virus(HCV) E2 Gene

  • 이영기 (한국인삼연초연구원 원료연구부) ;
  • 강신웅 (한국인삼연초연구원 원료연구부) ;
  • 김선원 (한국인삼연초연구원 원료연구부) ;
  • 박성원 (한국인삼연초연구원 원료연구부) ;
  • 이종철 (한국인삼연초연구원 원료연구부) ;
  • 이청호 (한국인삼연초연구원 원료연구부)
  • 발행 : 2001.12.01
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초록

Hog cholera virus(HCV) was purified from virus infected Bovine kidney cells. From this virus, total protein was analyzed by SDS-PAGE gel electrophoresis and about 55 kDa band of E2 envelope protein was detected. The viral RNA was purified and E2 cDNA was amplified by RT-PCR. E2 cDNA fragment was cloned to PCRII-TOPO cloning vector and named pE2. The analysis of nucleotide sequence showed that this E2 cDNA fragment inserted into pE2 was 1191 nucleotides long and coded 397 amino acids.

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참고문헌

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