YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
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Effect of Butanol Fraction of Mylabris phalerata on Induction of Apoptosis in U937 cells

반묘 BuOH층의 U937 세포주에 대한 apoptosis유도 효과

  • 허정은 (경희대학교 동서의학대학원) ;
  • 윤택준 (경희대학교 동서의학대학원) ;
  • 이종수 (대웅제약연구소) ;
  • 정진홍 (대전대학교 한의과대학) ;
  • 김성훈 (경희대학교 동서의학대학원)
  • Published : 2001.10.01
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Abstract

Mylabris phalerata(MP) is an insect that has been used for the treatment of cancer in oriental medicine. To evaluate the anticancer activity of Mylabris phalerata, We measured the cytotoxicity of Mylabris phalerata solvent fractions such as MC, EA, BuOH and residual layers on U937, human monocytic leukemia cells. Of those fractions BuOH layer of Mylabris phalerata was the most effective with ID$_{50}$ of 140$\mu\textrm{g}$/ml. It effectively caused DNA fragmentation from the concentration of 50$\mu\textrm{g}$/ml, showed apoptotic nucleus by tenets assay and expressed apototic portion stained by Annexin-V. It also induced the activation of caspase-3 and cleavage of the substrate poly (ADP-ribose) polymerase (PARP). These results suggest BuOH layer of Mylabris phalerata exerts anticancer activity by induction of apoptosis via activation of caspase-3 protease.e.

Keywords

References

  1. Apoptosis.Br.J.Cancer v.67 no.205 Wyllie,A.H.
  2. Annu.Rev.Physiol. v.60 no.619 The mitochnodrial death/life regulator in apoptosis and necrosis Kroemer,G.;Dallaporta,B.;Resche-Rigon,M.
  3. FASEB J. v.9 no.1277 The biochemistry of programmed celldeath Kroemer,G.;Petit,P.;Zamzami,N.;Vayssiere,J.L.;Mignotte,B.
  4. Am.J.Pathol. v.136 no.593 Apoptosis.The role of the endonuclease Arends,M.J.;Morris,R.G.;Wyllie A.H.
  5. Science v.281 no.1309 Mitochondria and apoptosis Green,D.R.;Reed,J.C.
  6. Mol.Cell.Biol. v.19 no.2986 Role for caspase-mediated cleavage of Rad51 in induction of apoptosis by DNA damage Huang,Y.;Nakada,S.;Ishiko,T.;Utsugisawa,T.;Datta,R.;Kharbanda,S.;Yoshida,K.;Talanian,R.V.;Weichselbaum,R.;Kufe,D.;Yuan,Z.M
  7. 中 腫瘤學,上冊 v.120 仁存
  8. 항종류본초도보 v.144 歐明;徐 華;李 文;駱和生
  9. J.Ethnopharn v.26 no.147 Medical uses of mylabris in ancient China and recent studies Wang,G.S.
  10. Exp.Cell.Res. v.256 no.12 Apoptotic DNA fragmentation Nagata,S.
  11. Bioche.Biophys.Acta. v.17 no.1387 Properties fo the caspases Stennick,H.R.;Salvesen,G.S.
  12. J.Biol.Chem. v.274 no.37450 DNA fragmentation factor 45-deficient cells are more resistant to apoptosis and exgibit different dying morphology than wild-type control cells Zhang,J.;Wang,X.;Bove K.;Xu,M.
  13. J.Biol.Chem. v.275 no.31199 Cytochrome C promotes caspase-9 activation by inducing nucleotide binding to Apaf-1 Jiang,X.;Wang,X.
  14. EMBO J. v.19 no.4310 Negative regulation of cytochrome C-mediated oligomerization of Apaf-1 and activation of procaspase-9 by heatshock Pandey,P.Saleh,A;Nakazawa,A.;Kumar,S.;Srinivasula,S.M.;Kumar,V.;Weichselbaum,R.;Kufe,D.;Kharbanda,S.