The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Biosynthetic Regulation of Inulinase from Bacillus sphaericus 188-1

Bacillus sphaericus 188-1이 생성하는 Inulinase의 생합성 조절

  • Kim, Na-Mi (Korea Ginseng & Tabacco Research Institute) ;
  • Lee, Jong-Soo (Department of Genetic Engineering and Bio-Medicinal RRC, Paichai University)
  • Published : 2001.02.01
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Abstract

Regulation of inulinase biosynthesis was studied in Bacillus sphaericus 188-1 Biosynthesis of inulinase was effectively induced in the presence of 0.5% inulin for 8 hrs. Fructose (0.5%) repressed the inulinase induction by inulin and as late as addition time of fructose, inulinase formation was decreased. Catabolite repression was not reduced by the addition of CAMP for 8 hrs of induction.

Inulinase의 생합성 조절 기작을 규명하여 이들의 대량생산을 위한 자료로 활용하고자 Bacillus sphae-ricus 188-1이 생성하는 inulinase의 생합성 조절에 관하여 조사하였다. Inulinase의 생합성은 0.5% inulin을 함유한 생합성 조절용액에서 8시간에 효율적으로 유도되었고 0.5% fructose의 첨가는 inulin에 의한 inulinase의 생합성 유도를 억제시켰으며 fructose를 늦게 첨가할수록 inulinase 생합성 유도가 낮아졌다. CAMP의 첨가는 catabolite repression을 감소시키지 못하였다.

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