Bioremediation of Diesel-Contaminated Soil by Bacterial Cells Transported by Electrokinetics

  • LEE, HYO-SANG (Department of Environmental Engineering and Biotechnology, Myongji University) ;
  • KISAY LEE (Department of Environmental Engineering and Biotechnology, Myongji University)
  • Published : 2001.12.01

Abstract

The electrokinetic technology was applied in bioremediation for the purpose of supplying a Pseudomonas strain capable of degrading diesel to contaminated soil bed, and their biodegradation of diesel was carried out after a desired cell distribution was obtained. Electrokinetic injection of the strain was made possible because the cells acted as negatively charged particles at neutral pH, and thus the cells were transported with a precise directionality through the soil mostly by the mechanism of electrophoresis and in part by electroosmosis. A severe pH change in the soil bed was formed due to the penetration of electrolysis products, which was harmful to the cell viability and cell transport. To achieve a desirable cell transport and distribution, the control of pH in soil bed by a recirculating buffer solution in electrode chambers was essential during the appliation of an electric field. The judicious selections of electrolyte concentration and conductivity were also important for achieving an efficient electrokinetic cell transport since a higher electrolyte concentration favored the maintenance of pH stability in soil bed, but lowered electrophoretic mobility on the other hand. With electrolyte solution of pH 7 phosphate buffer, a 0.05 M concentration showed a better cell transport buffer, a 0.05 M concentration showed a better cell transport than 0.02 M and 0.08 M. The cell under pH 8 were obtained, compared to the cells under pH 7 or pH 9 in a given time period Up to $60\%$ of diesel was degraded in 8 days by the Pseudomonas cell, which were distributed electrokinetically under the conditions of pH 8 ($1,800{\mu}S/cm$, a mixture of phosphate and ammonia buffers) and 40 mA in a soil bed of 15 cm length.

References

  1. Environ. Sci. Technol. v.27 Principles of electrokinetic remediation Acar, Y. B.;A. N. Alshawabkeh
  2. Chemtech v.1996 no.April Enhance soil bioremediation with electric fields Acar, Y. B.;M. F. Rabbi;E. E. Ozsu;G. J. Gale;A. N. Alshawbkeh
  3. J. Geotech. Geoenviron. Eng. v.123 Electrokinetic ijection of ammonium and sulfate ions into sand and kaolinite beds Acar, Y. B.;M. F. Rabbi;E. E. Ozsu
  4. J. Gen. Microbiol. v.136 The electrophoretic mobility of gram-positive and gram-negative bacteria Bayer, M. E.;J. L Sloyer, Jr.
  5. Surfactant Enhanced Electrokinetic Remediation of Gasoline Contaminated Soils Bhattacharya, S. J.
  6. J. Environ. Eng. v.123 Transport of nitrates through clay using electrokinetics Budhu, M.;M. Rutherford;G. Sills;W. Rasmussen
  7. Energy Sources v.19 Electrobioremediation of soils contaminated with hydrocarbons and metals: Progress report Chilingar, G. V.;W. W. Loo;L. F. Khilyuk;S. A. Katz
  8. J. Environ. Sci. Health v.A32 Surfactant-enhanced biodegradation of soil alkanes Churchill, P. F.;S. A. Churchill
  9. Bioremediation Engineering: Design and Application Cookson, Jr., J. T.
  10. J. Hazard. Mater. v.55 Electrokinetic transport of bacteria Deflaun, M. F.;C. W. Condee
  11. Environ. Technol. v.17 Electrokinetic supply of nutrients in soil bioremediation Elektrowicz, M.;V. Boeva
  12. Appl. Microbiol. Biotechnol. v.43 Use of Surfactants and slurrying to enhance the biodegradation in soils of compounds initially dissolved in nonaqueous-phase liquids Fu, M. F.;M. Alexander
  13. J. Geotech. Eng. v.112 Pb(Ⅱ) removal from kaolinite using electrokinetics Hamed, J. T.;Y. B. Acar;R. J. Gale
  14. Study of Electroosmosis in Soil: A Modified Theory and Its Application in Soil Decontamination Khan, L. I.
  15. J. Environ. Sci. Health v.A34 Effects of electric field directions on surfactant enhanced electrokinetic remediation of diesel-contaminated sandy soil Kim, J.;K. Lee
  16. J. Microbiol. Biotechnol. v.10 The possible involvement of the cell surface in aliphatic hydrocarbon utilization by an oil-degrading yeast Yarrowia lipolytica 180 Kim, T. H.;Y. S. Oh;S. J. Kim
  17. Environ. Sci. Technol. v.27 Electroreclamations: Applications in the Netherlands Lageman, R.
  18. Biotechnol. Bioprocess. Eng. v.4 Elctrokinetic transport of an NAPL-degrading microorganism through sandy soil Lee, H. S.;D. Jahng;K. Lee
  19. J. Contam. Hydrol. v.9 Hydrogen peroxide use to increase capacity for in situ bioremediation of contaminated soils and aquifers: A review Pardieck, D. L.;E. J. Bouwer;A. T. Stone
  20. Science v.260 Removal of conteminants from soils by electric field Probstein, R. F.;R. E. Hicks
  21. J. Environ. Eng. v.118 Electroosmotic contaminant-removal processes Segall, B. A.;C. J. Bruell
  22. Environ. Sci. Technol. v.27 Removal of contaminants from saturated clay by electroosmosis Shapro, A. P.;R. F. Probstein
  23. Introduction to Colloid and Surface Chemistry, $4^{th}$ed. Shaw, D. J.
  24. J. Microbiol. Biotechnol. v.9 Effects of various parameters on biodegradation of dgradable polymers in soil Shin, P. K.;E. J. Jung
  25. Handbook of Bioremediation Introduced organisms for subsurface bioremediation Thomas, J. M.;C. H. Ward;R. D. Norris(et al, ed.)
  26. Langmuir v.13 Electrokinetic potential of bacterial cells van der Wal, A.;M. Minor;W. Norde;A. J. B. Zehnder;L. Johannes
  27. Appl. Environ. Microbiol. v.61 Influence of nonionic surfactants on bioavailability and biodegradation of polycyclic aromatic hydrocarbons Volkering, F.;A. M. Breure;J. G. van Andel;W. H. Rulkens
  28. J. Hazard. Mater. v.57 The use of solid peroxides to stimulate growth of aerobic microbes in tundra White, M. W.;R. L. Irvine;C. R. Woolard
  29. Appl. Environ. Microbiol. v.60 Effect of a Pseudomonas rhamnolipid biosurfactant on cell hydrophobicity and biodegradation of octadecane Zhang, Y.;R. Miller