Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지

Characterization of Bone Marrow Cell Proliferating Arabinogalactan through Peyer`s Patch Cells from Rhizomes of Atractylodes lancea DC

  • Yu, Kwang-Won (Department of Kimchi and Food Science, Chonju National College of Science and Technology) ;
  • Hwang, Jong-Hyun (Department of Kimchi and Food Science, Chonju National College of Science and Technology)
  • Published : 2001.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

Bone marrow cell proliferating arabinogalactan-like polysaccharide (ALR-3IIa-1-1) has been purified from rhizomes of Atractylodes lancea DC. In order to characterize the essential structure of ALR-3IIa-1-1 for expression of the activity, sequential enzymatic digestion using ego-$\alpha$-L-arabinofurasidase (AFase) and ego-$\beta$-D-(1longrightarrow3)-galactanase (GNase) was employed. After ALR-3IIa-1-1 was digested with the AFase, the GNase digestion cleaved only 10% and 23% of 3-linked and 3,6-branched galactose, respectively, from arabinose-trimmed ALR-3IIa-1-1 (AT-ALR-3IIa-1-1), and gave small amounts of intermediate size (AT-G-2) and shorter oligosaccharides (AT-G-3) fractions in addition to a large amount of the GNase resistant fraction (AT-G-1). When AT-G-1 was redigested gradually with the AFase and GNase, it released trace amounts of oligosaccharides in addition to a large amount of the resistant fraction. When the final enzyme-resistant fraction from AT-G-1 was digested simultaneously with both AFase and GNase, the resistant fraction was significantly degraded into two long fragments (3AT-3G-1 and 2). The mixture of digestion products from the first GNase digestion of AT-ALR-3IIa-1-1 showed a significantly decreased bone marrow cell proliferation activity to about 30% of the activity of ALR-3IIa-1-1, but the GNase resistant fraction (AT-7-1) still had significant activity. Although the second gradual enzymatic digestion of AT-G-1 showed a marginal decrease in activity, the resulting fragments (3AT-3G-1 and 2) by the final simultaneous enzymatic digestion lost most of the activity. Component sugar, methylation and FAB-MS analyses indicated that the digestion products (AT-G-21 AT-G-31 2AT-2G-2 and 2AT-2G-3) released from AT-ALR-3IIa-1-1 by the sequential enzymatic digestion contained galactose-containing oligosaccharides mainly comprising 6-linked galactose, that some of which were partially arabinosylated, and these oligosaccharides were attached to $\beta$-D-(1longrightarrow3)-galactan backbone in its non-reducing terminal side as side chains.

Keywords

References

  1. J. Biol. Chem. v.257 Polysaccharide nomenclature, recommendation IUB-IUPAC Joint Commission of Biochemical Nomenclature(JCBN)
  2. Biogenesis of plant cell wall polysaccharides Carbohydrate polymers of plant cell walls Aspinasll, G.O.;Loewus, F.(ed.)
  3. Essays in biochemistry v.31 Arabinogalactan proteins : a family of cell--surface and extracellular matrix plant proteoglycans Bacic, A.;Du, H.;Stone, B.A.;Clarke, A.E.;Apps, D.K.(ed.)
  4. Plant Mol. Biol. v.30 Arabinogalactan proteins and plant differentiation Kreuger, M.;Holst, G.J.
  5. Mol. Immunol. v.22 Studies on polysaccharides from Angelica acutiloba IV. Characterization of anti-complementary arabinogalactan from Angelica acutiloba Kitagawa Yamada, H.;Kiyohara, H.;Cyong, J.C.;Otsuka, Y.
  6. Immunomodulating agents from plants Complement-activating polysac-charides from medicinal herbs Yamada, H.;Kiyohara, H.;Wagner, H.(ed.)
  7. Bioconj. Chem. v.5 Arabinogalactan for hepatic drug delivery Groman, E.V.;Enriquez, P.M.;Jung, C.;Josephson, L.
  8. Altern, Med. Rew. v.4 Larch arabinogalactan : clinical relevance of a novel immune-enhancing polysaccharide Kelly, G.S.
  9. Food Sci. Biotechnol Bone marrow cell proliferating fractions through Peyer's patch cells from rhizomes of Atractylodes lancea DC Yu, K.W.;Hwang, J.H.
  10. Phytochem. v.18 From and function of arabinogalactans and arabinogalactan-proteins Clarke, A.E.;Anderson, R.L.;Stone, B.A.
  11. Carbohydr. Res. v.243 The purification of commercially available endo- α-L-arabinosidase and α-L-arabinosidase for use in the structural analysis of pectic polysaccharides Leroge, P.;O'Neill, M.A.;Darvill, A.G.;Albersheim, P.
  12. J. Biol. Chem. v.265 Purification of an exo-β-D-(1-3)-galactanase of Irpex lacteus (Polyporus tulipiferae) and its action on arabinogalactan-proteins Tsumuraya, Y.;Mochizuki, N.;Hashimoto, Y.;Kovac, P.
  13. Anal. Chem. v.28 Colorimetric method for determination of sugars and related substances Dubois, M.;Gilles, K.A.;Hamilton, J.K.;Rebers, P.A.;Smith, F.
  14. Biochim. Biophys. Acta v.23 A new color reaction for the determination of aldopentose in presence of other saccharides Dische, D.;Borenfreund, E.
  15. Anal. Biochem. v.54 New method for quantitative determination of uronic acid Blumenkrantz, N.;Asboe-Hansen, G.
  16. Anal. Biochem. v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Bradford, M.M.
  17. Methods Enzymol. v.118 Isolation and characterization of plant cell walls and cell wall components York, W.S.;Darvill, A.G.;McNeil, M.;Stevenson, T.T.;Albersheim, P.
  18. Anal. Biochem. v.148 Quantification of arabinogalactan-protein in plant extracts by single radial gel diffusion Holst, G,J.;Clarke, A.E.
  19. Korean J. Food Sci. Technol. v.33 Bone marrow cell proliferation activity through intestinal immune system by the components of Atractylodes lancea DC Yu, K.W.;Shin, K.S.
  20. J. Biochem. (Tokyo) v.55 A rapid permethylation of glycolipid, and polysaccharide catalyzed by methylsulfiny1 carbanion in dimethyl sulfoxide Hakomori, S.
  21. Carbohydr. Res. v.123 Determination by methylation analysis of the glycosyl linkage compositions of microgram quantities of complex carbohydrates Waeghe, T.J.;Darvill, A.G.;McNeil, M.;Albersheim, P.
  22. Carbohydr. Res. v.219 Heterogeneity and characterization of mitogenic and anti-complementary pectic polysaccharides from the roots of Glycyrrhiza uralensis Fisch et DC. Zhao, J.F.;Kiyohara, H.;Yamada, H.;Takemoto, N.;Kawamura, H.
  23. Carbohydr. Res. v.40 Quantitative analysis by various G.L.C. response-factor theories for partially methylated and partially ethylated alditol acetates Sweet, D.P.;Shapiro, R.H.;Albersheim, P.
  24. Phytomed. v.5 Enhanced production of hematopoeitic growth factors through T cell activation in Peyer's patches by oral administration of Kampo (Japanese herbal) medicine Juzen-Taiho-To Hong, T.;Matsumoto, T.;Kiyohara, H.;Yamada, H.
  25. Int. J. Oncol. v.3 A new fluorometric assay for cytotoxicity measurements in vitro. Page, B.;Page, M.;Noel, C.