Effect or $\gamma$-Radiation on Growth and Antioxidant Enzyme Activities in Red Pepper

방사선처리가 고추의 생육과 항산화 효소의 활성에 미치는 영향

  • 권순태 (안동대학교 생명자원과학부) ;
  • 정은아 (안동대학교 생명자원과학부) ;
  • 김재성 (한국원자력연구소 동위원소. 방사선응용연구팀)
  • Published : 2001.12.01

Abstract

Seedlings of red pepper (Capsicum annum L.) were exposed to various doses of ${\gamma}$-radiation )($^{60}$ Co)and cultivated in the 1/2,000 a pot. Plant hight the number of leaves, flowers and fruits, chlorophyll contents, plant dry weight, ac- tivites of antiozidant enzymes such as superoxide dismutase(SOD) adn peroxidase(POD), were determined. Plant height of seedling treated with 1 Gy of ${\gamma}$-radiation was increased up to 16.6% as compared to untreated control at 80 days after treatment, while those treated with 20 Gy or 50 Gy was decreased 22.0% or 75.0% respectively. The plants treated with 1 Gy or 5 Gy were increased the number of leaves up to 41.2% and 24.9% as well as dry weight of fruit up to 58.4% and 49.4% respectively. However treatment of radiation higher than 20 Gy significantly inhibited the growth, chlorophyll content and yield of pepper fruit. Activities of antioxidant enzymes, SOD and POD were tem-porary increased within one or five days after treatment depending on the doses of treatment , and the activities were gradually decreased to control level of lower thereafter. The SOD and POD activities in the leaves of pepper plant treated with 50 Gy were 5.5 and 6.0folds higher than that of control at five days after treatment, respectively.

고추의 유모에 감마 방사선($^{60}$Co)을 조사하여 식물의 생육, 수량, 엽록소 함량및 항산화효소, superoxide dismutase (SOD) 및 peroxidase(POD)의 활성에 미치는 영향을 조사하였다. 고추의 초장은 1 Gy 처리에 의해 무처리보다 16.6%가 증가하였은 고선량인 20Gy 및 50Gy 처리에 의해서 는 각각 22.0 및 75.0%가 감소하였다. 잎의 수는 1Gy 및 5Gy처리에서 각각 41.2%및 24.9%가 증가하였으나 20Gy이상의 고선량에서는 감소하였고, 과실은 1및 5Gy 처리에의해 착과수가 증가하였고, 건물중 기준으로 각각 58.4및 49.4%가 증가하였다. 저선량의 방사선의 처리는 고추의 엽록소함량에 영향을 미치지 않았으나,증가된 활성은 다시 감소하여 처리후 20일에는 무처리와 비슥하거나 낮았다. 50Gy를 처리한 후 5일째에 측정한 SOD 및 POD의 효소 활성은 무처에 비하여 각각 5.5 및 6.0배가 증가하였다.

Keywords