Abstract
Phytolalexins are produced in plants affected by various environmental factors such as fungal infection treatment with many chemicals and irradiation by ultraviolet light. When pepper and tobacco bel suspension cultures were grown on a basal MS medium supplemented with 2,4-D(1mg/$\ell$, benzyl adenine(0.001 mg/$\ell$) and 100$\mu$ M jasmonic acid, the production of capsidiol was observed. The total of compound found in pepper plant were around seventy and thirty of them were located intissue-specific manner. 1-propanethiol, $\alpha$-D-xylofuranoside, phenol, hexadecanonic acid ethyl tridecanoate, phytol, linoleic acid and capsidiol are those which have change the production level by treatments, such as the inoculation of Phytophthora capsici Leonian, the metalaxyl treatment and the UV-B irradiation, respectively. The content of capsidiol on inoculation of P. capsici with metalxyl suspension in soil were higher than those of P.capsici without metalaxyl. When the soil dernch of metalaxyl treatment (1$\mu\textrm{g}$/${mu}ell$)was delayed after inoculation, the content of capsidiol were higher than that of before. Irrradiated UB-B the production on capsidiol was identified only at leaf, and contents were the highest for 24 hrs incubation after 20 minutes irradiation.
역병균 접종, UV-B 조사 등의 환경인자에 의해 고추의 생체방어물질 및 유도되는 화합물을 조사하엿다. Capsidiol은 2,4-D(1mg/$\ell$), benzyl adenine (0.001 mg/$\ell$)이 첨가된 MS배지에서 jasmonic acid 100$\mu$M을 처리한 담배와 고추 현탁배양세포의 추출물로 부터 Rf 0.32(TLC)에서 동정되었다. 고추식물체내에서 70개의화합물이 동정되었고, 그중 부위별 차이가 있는 성분은 30개 이었다. 역병균 접종, metalaxyl 처리, UV-B 조사 등과 같은 환경인자에 의하여 식물체 중의 1-propanethiol, $\alpha$-D-xylofur-anoside, phenol, hexadecanoni acid, linoleic acid, phytol, ethyl tridecanoate 및 capsidiol 등의 생성 성분 함량 간에 차가 있었다. Capsidiol은 역병균 접종, metalayl과 역병균 혼용 접종 및 UV-B 조사에 의해 생성되었다. capsidiol 함량은 역병균 단독 접종에 비하여 metalaxyl 혼용 처리가 높았고, 역병균 접종 전 metalaxyl(1${\mu}\ell$/$m\ell$)처리시보다 접종 후 처리시가 높았다. UV-B 조사시의 capsidiol은 잎에서 생성되었고, 특히 UV-B 20분조사후 24시간 incubationgon 했을때 가장 높았다.
