Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Fabrication and Characterisation of a Novel Pellicular Adsorbent Customised for the Effectvie Fluidised Bed Adsorption of Protein Products

  • Sun, Yam (Department of Biochemical Engineering , Tianjin University, Tianjin 30072, P. R. China) ;
  • Pacek, Andrzej W. (Biochemical Recovery Group Centre for Formulation Engineering, School of Engineering(Chemical Engineering) University of Birmingham Edgbaston, Birmin) ;
  • Nienow, Alvin W. (Biochemical Recovery Group Centre for Formulation Engineering, School of Engineering(Chemical Engineering) University of Birmingham Edgbaston, Birmin) ;
  • Lyddiatt, Andrew (Biochemical Recovery Group Centre for Formulation Engineering, School of Engineering(Chemical Engineering) University of Birmingham Edgbaston, Birmin)
  • Published : 2001.12.01
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Abstract

A dense pellicular solid matrix has been fabricated by coating 4% agarose gel on to dense zironia-silica(ZS) spheres by watr-in-oil emulsification . The agarose evenly laminated the ZS bead to a depth of 30㎛, and the resultin gpellicular assembly was characterised by densities up to 2.39g/mL and a mean particle dimeter of 136 ㎛. In comparative fluidisation tests, the pellicular solid phase exhibited a two-fold greater flow velocity than commercial benchmark ad-sorbents necessary to achieve common values of bed expansion. Furthermore, the perlicular parti-cles were characterised by improved qualities of chromatographic behaviour, particularly with re-spect to a three-fold increase in the apparent effective diffusivity of lysozyme within a pellicular assembly modified with Cibacron Blue 3GA. The properties of rapid protein adsorption/desorp-tion were attributed to the physical design and pellicular deployment of the reactive surface in the solid phase. When combined with enhanced feedstock throughput, such practical advantages recommend the pellicular assembly as a base matrix for the selective recovery of protein products from complex, particulate feedstocks(whole fermentation broths, cell disruptates and biological extracts).

Keywords

References

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