Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Counterion Effects on Transection Activity of Cationic Lipid Emulsion

  • Kim, Young-Jin (Biomedical Research Center, Korea Institute of Science and Technology, ) ;
  • Kim, Tae-Woo (Biomedical Research Center, Korea Institute of Science and Technology, ) ;
  • Hesson Chung (Biomedical Research Center, Korea Institute of Science and Technology, ) ;
  • Kwon, Ik-Chan (Biomedical Research Center, Korea Institute of Science and Technology, ) ;
  • Jeong, Seo -Young (Biomedical Research Center, Korea Institute of Science and Technology, )
  • Published : 2001.07.01
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Abstract

Cationic lipid emulsion system consisting of 1, 2-dioleoyl-sn-slycero-3-trimethyl-ammonium-propane(DOTAP) and plasmin DNA with various counterions in the lipid headgroups were prepared. The transfection activity of the cationic lipid emulsion systems was then investigated in vitro and in vivo. The complex formation of plasmid DNA lipid emulsion was affected by the counterions through charged headgroup repulsion and also by the salt concen-tration in the media. As such , the transfection activity of the DOTAP emulsion system can be controlled by changing the counterions.

Keywords

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