Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Evaluation of Bioremediation Effectiveness by Resolving Rate-Limiting Parameters in Diesel-Contaminated Soil

  • Joo, Choon-Sung (Department of Environmental Engineering and Biotechnology, Myongji University) ;
  • Oh, Young-Sook (Department of Environmental Engineering and Biotechnology, Myongji University) ;
  • Chung, Wook-Jin (Department of Environmental Engineering and Biotechnology, Myongji University)
  • Published : 2001.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

The biodegradation rates of diesel oil by a selected diesel-degrading bacterium, Pseudomonas stutzeri strain Y2G1, and microbial consortia composed of combinations of 5 selected diesel-degrading bacterial were determined in liquid and soil systems. The diesel degradation rate by strain Y2G1 linearly increased $(R^2=0.98)$ as the diesel concentration increased up to 12%, and a degradation rate as high as 5.64 g/l/day was obtained. The diesel degradation by strain Y2G1 was significantly affected by several environmental factors, and the optimal conditions for pH, temperature, and moisture content were at pH8, $25^{\circ}C$, and 10%, respectively. In the batch soil microcosm tests, inoculation, especially in the form of a consortium, and the addition of nutrients both significantly enhanced the diesel degradation by a factor of 1.5 and 4, respectively. Aeration of the soil columns effectively accelerated the diesel degradation, and the initial degradation rate was obviously stimulated with the addition of inorganic nutrients. Based on these results, it was concluded that the major rate-limiting factors in the tested diesel-contaminated soil were the presence of inorganic nutrients, oxygen, and diesel-degrading microorganisms. To resolve these limiting parameters, bioremediation strategies were specifically designed for the tested soil, and the successful mitigation of the limiting parameters resulted in an enhancement of the bioremediation efficiency by a factor of 11.

Keywords

References

  1. Int. Biodet.Biodeg. v.39 Bioremediation of organic waste site: A critical review of microbiological aspects Alard, A. S.;A. H. Neilson
  2. Biodegradation and Bioremediation Nonaqueous-phase liquids and compounds with low water solubility Allexander, M.
  3. Environ. Toxicol. Chem. v.11 Surfactants at low concentrations stimulate biodegradation of sorbed hydrocarbons in samples of aquifer sands and soil slurries Aronstein,B.N.;M. Alexander
  4. Annual Book of ASTM Standards v.73 ASTM
  5. Crit. Rev. Microbiol. v.5 Stimulated petroleum biodegradation Atlas, R. M.
  6. J. Chem. Technol. Biotechnol. v.52 Microbial bydrocarbon degradation Bioremediation of oil spills Atlas, R. M.
  7. Advances in Microbial Ecology v.12 Hydrocarbon biodegradation and oil spill bioremediation Atlas, R.M.;R. Bartha;K.C. Marshall(ed.)
  8. J. Microbiol. Biotechnol. v.7 Degradation of chlorophenols and phenol mixtures by cooperative activities of chlorophenol-degrading strains Bae, H.-S.;Y.-G. Cho;S.-T. Lee
  9. Microb. Ecol. v.12 Biotechnology of petrolem pollutant biodegradation Bartha, R.
  10. Long-Term Environmental Effects of Offshore Oil and Gas Development Transport and transformations of petroleum: Biological processes Bartha, R.;R.M. Atlas;F. Boesch(eds.);N.N. Rabalais(eds.)
  11. The Release of Genetically Modified Microorganisms Bioremediation and waste management Bewley,R.J.;D. E. S. Tull(eds.);M. Sussman(eds.)
  12. Petroleum Microbiology The fate of petroleum in soil ecosystems Bossert, I.;R. Bartha;R.M. Atlas(ed.)
  13. Nature v.368 Effectiveness of bioremediation for the Exxon Valdez oil spill Bragg, J.R.;R.C. Prince;E.J. Harner;R.M. Atlas
  14. Can. J. Microbiol. v.43 Bacterial degradation of emulsified crude oil and the effect of various surfactants Bruheim, P.;H. Bredholt;K. Eimhjellen
  15. Chemosphere v.33 Effect of a dichlorophenol-adapted consortium on the dechlorination of 2,4,6-trichlorophenol and pentachlorophenol in soil Chang, B.V.;L.N. Yeh;S.Y. Yuan
  16. Proceedings of the 1991 Oil Spill Conference Bioremediation technology development and application to the Alaskan spill Chianelli R. R.;R. Aczel;R.E. Bare;G.N. Geroge;M.W. Genowitz;M.J. Grossman;C.E. Haith;F.J. Kaiser;R.R. Lessard;R. liotta;R. L. Mastracchio;V. Minak-Bernero;R.C. Prince;W.K. Robbins;E.I. Stiefel;J.B. Wilkinson;S.M. Hinton
  17. Waste Manag v.15 Surfactant-enhanced bioremediation Churchill, P.F.;R. J. Dudley;S. A. Churchill
  18. Progress Report Abstracts Hydrocarbonoclastic repository Cobet, A. B.
  19. Appl. Environ. Microbiol. v.47 Production of a biosurfactant from Torulopsis bombicola Cooper, D.G.;D. A. Paddock
  20. Appl. Microbiol, Biotechnol. v.46 Adding sodium dodecyl sulfate and Pseudomonas aeruginosa UG2 biosurfactants inhibits polycylic aromatic hydrocatbon biodegradation in a weathered creosotecontaminated soil Deschenes, L.P.;J.P. Lafrance;J. Villeneuve;R. Sanson
  21. Appl. Environ. Microbiol. v.37 Effect of environmental parameters on the degradation of oil sludge Dibble, J.T.;R. Bartha
  22. Environ. Sci. Technol. v.25 Solubilization of polycyclic aromatic hydrocarbons in micellar nonionic surfactant solutions Edwards, D.A.;R. G. Luthy;Z. Liu
  23. Environ Sci. Technol. v.25 Sediment-and saturated-soil-associated reactions invilving an anionic surfactant (dodecylsulfate).2. Partition of PAH compounds among phases Jafvert, C.T.
  24. Yarrowia lipolytica 180 J. Microbiol. v.37 Identificaton and characterization of an oil-degrading yeast Kim, T.-H.;J.-H. Lee;Y.-S. Oh;K.S. Bae;S.-J. Kim
  25. Environ. Sci. Technol. v.25 Inhibition of phenanthrene mineralization by nonionic surfactants in soil-water systems laha, S.;R. G. Luthy
  26. Biotechnol. Bioeng. v.40 Effects of nonionic surfactants on the solubilization and mineralization of phenanthrene in soil-water systems Laha, S.;R. G. Luthy
  27. Arctic Alpine Res. v.30 Oil biodegradation potential in alpine habitats Margesin, R.;F. Schinner
  28. Appl. Microbiol. Biotechnol v.32 Effect of added microbial surfactants on hydrocarbon degradation with a soil population in a stirred reactor Oberbremer, A.;R. Muller-Hurtig;F. Wagner
  29. Biotech. Lett v.22 Biological treatment of oil-contaminated sand: Comparison of oil degradation based on thin-layer chromatography/flame ionization detector and respirometric analysis Oh, Y.-S.;W.-Y. Choi;Y.-H. Lee;S.-C. Choi;S.-J. Kim
  30. Experimental Soil Microbiology Pramer, D.;E. L. Schmidt
  31. Humic Substances in the environment Schnitzer, M.;S.U. Khan
  32. J. Microbiol. Biotechnol. v.9 Effects of various parameters on biodegradation of degradable polumers in soil Shin, P.K.;E. J. Jung
  33. Appl. Environ. Microbiol. v.56 Effects of jet fuel spills on the microbial community of soil Song. H.-G.;R. Bartha
  34. Appl. Environ. Microbiol. v.65 Bacterial adhesion to soil contaminants in the presence of surfactants Stelmack, P.L.;M.R. Gray;M. A. Pickard
  35. Experimental Microbial Ecology The soil environment Clay-humus-microbe interactions Stotzky, G.;R. G. Burns;R. G. Burns(eds.);J. J. Slater(eds.)
  36. Chemosphere v.29 Biodegradation of anionic surfactants in the presence of petroleum contaminants Sundaram, N.S.;M. Sarwar;S. S. Bang;M. R. Islam
  37. Appl. Environ. Microbiol. v.60 Degradation of polycyclic aromatic hydrocarbons in the presence of synthetic surfactants Tiehm, A.
  38. DECHEMA Biotechnology Conference Bioavailability of polycyclic aromatic hydrocarbons - solubilizing potential and biological effeciency of technical surfactants Tiehm, A.;W. G. Zumft
  39. Outlook agric v.17 Microbial decomposition of herbicides in soil Torstensoon, L.
  40. Guidelines Establishing Test Procedures for the Analysis of Pollutants under Clean Water Act; Final rule and Interim Final Rule and Proposed Rule U. S. EPA
  41. Environmental Monitoring Systems Laboratory Interlaboratory Comparison Study; Methods for Volatile and Semi-Volatile Compounds U.S. EPA
  42. Environ. Pollut v.67 Use of selected autochthonous soil bacteria to enhance degradation of hydrocarbons in soil Vecchioli, G.I.;M. T. Delpanno;M.T. Painceira
  43. Appl. Environ. Microniol. v.61 Influence of nonionic surfactants on bioavailability and biodegradation of polycyclic aromatic hydrocarbons Volkering, F.;A.M. Breure;J.G. van Andel;W.H. Rulkens
  44. Chemosphere v.41 Biodegradation of polycyclic aromatic hydrocarbones by a mixed culture Yuan, S.Y.;S. H. Wei;B. V. Chang
  45. Appl. Environ. Microbiol. v.58 Enhanced octadecane dispersion and biodegradation by a Pseudomonas rhamnolipid surfactant (biosurfactant) Zhang, Y.;R.M. Miller