대한미생물학회지 (The Journal of the Korean Society for Microbiology)

대한미생물학회 (The Korea Society for Microbiology)
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결핵균 감염에 의한 THP-1 세포에서의 Prothymosin alpha 유전자 발현증가

Up-regulation of Prothymosin alpha in THP-1 Cells Infected with Mycobacterium tuberculosis

  • 송호연 (순천향대학교 의과대학 미생물학교실) ;
  • 장광식 (순천향대학교 의과대학 미생물학교실) ;
  • 변희선 (순천향대학교 의과대학 미생물학교실) ;
  • 이신제 (순천향대학교 의과대학 미생물학교실) ;
  • 김진구 (생명공학연구소) ;
  • 최용경 (생명공학연구소) ;
  • 고광균 (순천향대학교 의과대학 미생물학교실)
  • Song, Ho-Yeon (Department of Microbiology, Soonchunhyang University College of Medicine) ;
  • Jang, Kwang-Sik (Department of Microbiology, Soonchunhyang University College of Medicine) ;
  • Byoun, Hee-Sun (Department of Microbiology, Soonchunhyang University College of Medicine) ;
  • Lee, Shin-Je (Department of Microbiology, Soonchunhyang University College of Medicine) ;
  • Kim, Jin-Koo (Korea Research Institute of Bioscience & Biotechnology) ;
  • Choe, Yong-Kyung (Korea Research Institute of Bioscience & Biotechnology) ;
  • Ko, Kwang-Kjune (Department of Microbiology, Soonchunhyang University College of Medicine)
  • 발행 : 2000.04.30
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초록

Mycobacterium tuberculosis is capable of growing and survival within macrophage. The purpose of this study was to identify the genes regulated by infection of mycobacteria in human monocytic THP-1 cells. We used the differential display reverse transcriptase polymerase chain reaction (DD RT-PCR) and nothern blot analysis to confirm the differentially expressed genes from THP-1 cells infected with live Mycobacterium tuberculosis H37Rv, heat-killed Mycobacterium tuberculosis H37Rv and live Mycobacterium bovis BCG. Among many up or down-regulated clones, 27 clones were sequenced and compared with known genes on GenBank. Thirteen of over-expressed clones from THP-1 cells infected with live Mycobacterium tuberculosis H37Rv were identical to human prothymosin alpha, eight were novel clones and six clones showed homology with Human ferritin H chain, Esherichia coli bgl, Mouse RNA-dependent EIF-2 alpha kinase, E. coli htrL, Hyaluronan receptor and T cell receptor. Our result suggests that Mycobacterium tuberculosis might regulate prothymosin alpha gene transcription in monocytic THP-1 cell.

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