Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Increased Poly(3-Hydroxybutyrate) Accumulation in Recombinant Escherichia coli from Whey by Agitation Speed Control

  • Kim, Beom-Soo (Department of Chemical Engineering, Konyang University) ;
  • Brian K. O'Neill (Department of Chemical Engineering, University of Adelaide) ;
  • Lee, Sang-Yup (Department of Chemical Engineering, Korea Advanced Institute of Science and Technology)
  • Published : 2000.10.01
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Abstract

The timing of poly(3-Hydroxybutyrate) (PHB) biosynthesis was controlled by varying the agitation speed of a stirred tank fermentor during the pH-stat fed-batch culture of recombinant Escherichia coli strain GCSC 6576 harboring pSYL107. Using a concentrated whey solution containing ca. 200 g/l lactose as the nutrient feed, the PHB content was only 57% after 35h due to volumetric limitation of the fermentor. However, by limiting the oxygen by maintaining the agitation speed at 300 rpm, the final PHB content increased to 70% after 70h with a cell concentration of 15 g/l. When the agitation speed was increased up to 500 rpm, a cell concentration of 31 g/l with 80% PHB was obtained after 52h. A further increase in the maximum agitation speed increased the cell concentration, PHB concentration, and PHB productivity, however, the PHB content decreased to 56-58%.

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References

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