Journal of Sericultural and Entomological Science (한국잠사곤충학회지)

Korean Society of Sericultural Science (한국잠사학회)
권호 표지

Cryopreservation of Mulberry(Morus) Seeds in Liquid Nitrogen(L$N_2$)

건조 및 초저온 처리에 의한 뽕나무 종자의 장기 보존

  • 최영철 (농업과학기술원 잠사곤충부) ;
  • 류근섭 (경북대학교 농과대학) ;
  • 방혜선 (농업과학기술원 잠사곤충부)
  • Published : 2000.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

To investigate the possibility of crypreservation of mulberry seeds in liquid nitrogen(LN$_2$), characteristics of the seeds were examined after picking mulberry syncarps and drying-heat treatment. Storage in LN$_2$has the potential of providing indifinite preservation of valuable seed germplasm. Determining the tolerance of seeds among given cultivars to LN$_2$cooling and subsequent rewarming is the first step to establishing the feasibility of LN$_2$storage. Seeds of 4 mulberry varities were treated to LN$_2$(-196$\^{C}$) for 24 hours after drying heat treatment. Seed moisture content of Daeryukppong was the highest. As moisture content of mulberry seed was below 1%, storage in LN$_2$was safe. And drying heat treatment for 60 minutes was suitable to prevent decreased germination rate and germination vigor of seeds. The seeds of Cheongilppong were unsuibable to cryopreserve in LN$_2$for longterm storage.

Keywords

References

  1. J. Kor. Soc. Hort. Sci. v.35 no.5 Survival by vitri-fication of shoot tips of Chrysanthemum mori-folium L. cooled to -195℃ Ahn, Y. H.;A. Sakai
  2. Cr. hebd. Seanc. Acad. Sci. Paris v.181 Sur ra suspension momentanee de la vie chez certaines graines Beequerel, P.
  3. Arabidopsis information service v.14 Cryopreservation of Arabidopsis thaliana and other seeds by storage in liquid nitrogen Gresshoff, P.;E. Gartner
  4. J. natn. Inst. agric. Bot. v.10 Seed deterioration in relation to storage conditions and it's influence upon germination, chromosomal damage and plant performance Harrison, B. J.
  5. Seed Sci. and Technology v.5 Storage of Al-lium cepa seed at low temperatures Harrison, B. J.;R. Carpenter
  6. Ann. Botany v.37 A Method of recording germination curves Janssen, J. G. M.
  7. Physiol. Plant v.21 Effect of soil salinity and growth regulators on germination and seeding metabolism of water Kotowski, T. T.;M. N. Sarin;A. Narayanan
  8. Ann. Bot.(London) v.42 Germination and liquid nitrogen storage of Cassva seed Munford, D. M.;B. W. Grout
  9. Plant Cell, Tissue and Organ Culture v.27 Cryopreservation of in vitro grown shoot tips of apple and pear by vitrification Niino, T.;A. Sakai;S. H. Yakuwa;K. Nojiro
  10. Nature(London) v.164 no.666 Revival of spermatozoa after vitrification and dehydration at low temperature Polge, C.;A. U. Smith;A. S. Parker
  11. Cryobiology v.27 no.657 A simple and efficient procedure for cryopreservation of nuclear cells of navel orange by vitrification Sakai, A.;S. Kobayashi
  12. Some factors contributing the survival of crop seeds coolde to temperature of liquid nitrogen. In crop genetic resources for today and tomorrow Sakai, A.;Noshino;O. H. Frankel(ed.);J. G. Hawkes(ed.)
  13. J. Seed Technol. v.5 no.1 Tolerance of crop seeds to cooling and storage in liquid nitrogen Stanwood, P. C.
  14. Plant cols hardiness and freezing stress Ultracold preservation of seed germplasm Stanwood, P. C.;L. N. Bass;P. Li(ed.);A. Sakai(ed.)
  15. Seed Sci. and Technol. v.9 Seed germplasm preservation using liquid nitrogen Stanwood, P. C.;L. N. Bass
  16. Hort-science v.14 Seed storage of several horticultural species in liquid nitrogen (-196℃) Stanwood, P. C.;E. E. Ross
  17. Plant Cell Rep. v.9 Crypreservation of isolated mint shoot tips by vitrification Towill, L. E.
  18. Plant Physiol. v.53 Seed aging; Chrmosome stability and extend viability of seeds stored fully imbibed Villers, T. A.