Biotechnology and Bioprocess Engineering:BBE

  • 제5권6호
  • /
  • Pages.418-421
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  • 2000
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  • 1226-8372(pISSN)
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  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Direct Colorimetric Assay of Microcystin Using Protein Phosphatase

  • Oh, Hee-Mock (Environmental Bioresources Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Seog-June (Environmental Bioresources Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Jee-Hwan (Environmental Bioresources Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Chan-Sun (Environmental Bioresources Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Yoon, Byung-Dae (Environmental Bioresources Laboratory, Korea Research Institute of Bioscience and Biotechnology)
  • 발행 : 2000.11.01
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초록

A new direct colorimetric assay of microcystin in water and algal samples is proposed consisting of two procedures as follows: 1) the elimination of phosphorus in the sample and concentration of microcystin using a C(sub)18 cartridge, 2) the detection of the released phosphorus by the ascorbic acid method and determination of protein phosphatase (PP) inhibition by microcystin. The optimum amounts of phosphorylase ${\alpha}$ and PP-1 in 50 ${\mu}$L concentrated sample were 50$\mu\textrm{g}$/50${\mu}$L buffer and 1.0unit/50${\mu}$L buffer, respectively, for the best assay. The pH for the maximum activity of PP-1 was 8. The minimum detectable concentration for this method was about 0.02$\mu\textrm{g}$/L, which is sufficient to meet the proposed guideline level of 1$\mu\textrm{g}$ microcystin/L in drinking water. Consequently, it would seem that the proposed direct colorimetric assay using PP is a rapid, easy, and convenient method for the detection of microcystin in water and algal samples.

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참고문헌

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