Biotechnology and Bioprocess Engineering:BBE

  • 제5권5호
  • /
  • Pages.372-378
  • /
  • 2000
  • /
  • 1226-8372(pISSN)
  • /
  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Development of Cell Line Preservation Method for Research and Industry Producing Useful Metabolites by Plant Cell Culture

  • Cho, Ji-Suk (Department of Biological Engineering) ;
  • Chun, Su-Hwan (Department of Biological Engineering) ;
  • Lee, Song-Jae (Department of Biological Engineering) ;
  • Kim, Ik-Hwan (Graduate School of Biotechnology, Korea University) ;
  • Kim, Dong-Il (Department of Biological Engineering and Institute of Industrial Biotechnology, Inha University)
  • 발행 : 2000.09.01
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초록

The cell culture of Angelica gigas Nakai producing decursin derivatives and immunostimulating polysaccharides was preserved in liquid nitrogen after pre-freezing in a deep freezer at -70$^{\circ}C$ for 480 min. The effects of the cryoprotectant and pretreatment before cooling were investigated to obtain the optimal procedure for cyropreservation. When compared to mannitol, sorbitol, or NaCl with a similar osmotic pressure, 0.7 M sucrose was found to be the best osmoticum for the cryopreservation of A. gigas cells. In the pre-culture medium, the cells in the exponential growth phase showed phase showed the best post-freezing survival after cryopreservation. A mixture of sucrose, glycerol, and DMSO was found to be an effective cryoprotectant and a higher concentration of the cryoprotectant provided better cell viability. When compared with the vitrification, the optimum cryopreservation method proposed in this study would seem to be more effective for the long-term storage of suspension cells. The highest relative cell viability established with the procedure was 89%.

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참고문헌

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