KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Characteristics of Cellulose Production by Acetobacter sp. A9 in Static Culture

정치배양에서 Acetobacter sp. A9에 의한 셀룰로오스 생산특성

  • Published : 2000.12.01
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Abstract

The optimum fermentation conditions for the production of cellulose by a newly isolated Acetobacter sp. A9 were determined in static cultures. The strain was able to produce cellulose at $25-30^{\circ}C$ with a maximum at $30^{\circ}C$. Cellulose production occurred at pH 6.5-8.0 with a maximum at pH 6.5. The optimal culture medium was found to consists of 1.0% glucose, 1.0% yeast extract, 0.7% polypeptone, 0.15% acetic acid and 0.02% succinic acid. Cellulose production by Acetobacter sp. A9 followed the growth curve. Highest cellulose production, under optimum conditions, was $24.1m^2$, although this strain typically produced only $12.1 g/m^2$ in the basic medium. Cellulose production also depended on the depth and volume of the medium.

부패된 사과로부터 새롭게 분리된 Acetobacte sp. A9에 의 한 Be 생산조건을 정치배양에 의하여 검토하였다. 본 균주는 2 $25-30^{\circ}C$에서 BC를 생산할 수 있었으며, $30^{\circ}C$에서 최대 생산 능을 나타내었다. 또한 본 균주는 배지의 초기 pH 6.5-8.0에 서 BC를 생산할 수 있었으며, pH 6.5에서 최대 생산능을 나타내었다. BC 생산올 위한 최적 배지조건은 glucose 1.0%, y yeast extract 1.0%, polypeptone 0.7%, acetic acid 0.15% 및 succinic acid 0.02 %였다. Acetobacter sp. A9에 의한 BC 생산 은 "growth-associated type"이었다. 최적 배양조건하에서 배양 6일만에 최대 $24.1 g/m^2$ 의 BC가 생산되어 기본배지보다 약 2배의 생산성 향상이 이루어졌다.. BC 생산능은 배지의 표면 적보다는 배지량과 배지의 깊이에 크게 영향올 받았다.

Keywords

References

  1. J. Gen. Microbiol. v.21 Cellulose production by Acetobacter acetigenum and other Acetobacter spp. Dudman, W. F.
  2. TIBTECH v.16 Novel and estabilished applications of microbial polysaccharides Sutherland, I. W.
  3. The Plant Cell v.7 Cellulose biosynthesis Delmer, D. P.;Y. Amor
  4. Microbiol. Rev. v.55 Cellulose biosynthesis and function in bacteria Ross, P.;R. Mayer;M. Benziman
  5. J. Fermen. Bioeng. v.81 Cellulose production by Acetobacter pasteurianus on silicone membrane Yoshino, T.;Y. Asakura;K. Toda
  6. J. Gen. Microbiol. v.128 Cellulose-negative mutants of Acetobacter xylinum Valla, S.;J. Kjosbakken
  7. Appl. Biochem. Biotech. v.24/25 Acetobacter cellulose pellicle as a temporary skin substitute Fontana, J. D.;A. M. De Souza;C. K. Fontana;I. L. Torriani;J. C. Moreschi;B. J. Gallotti;S. J. De Souza;G. P. Narcisco;J. A. Bichara;L. F. X. Farah
  8. J. Mat. Sci. v.25 The structure and mechanical properties of sheets prepared from bacterial cellulose Nishi, Y.;S. Yamanaka;K. Watanabe;N. Kiamura;M. Iguchi;S. Mitsuhashi
  9. Biosci. Biotech. Biochem. v.61 High rate production in static culture of bacterial cellulose from sucrose by a newly isolated Acetobacter strain Kojima, Y.;A. Seto;N. Tonouchi;T. Tsuchida;F. Yoshinaga
  10. Kor. J. Appl. Microbiol. Biotechnol. v.28 Isolation and identification of cellulose-producing bacteria Son, H. J.;O. M. Lee;Y. G. Kim;S. J. Lee
  11. Food Hydrocolloids v.10 Isolation and partial characterization of cellulose produced by Acetobacter xylinum Embuscado, M. E.;J. N. BeMiller;J. S. Marks
  12. J. Fermen. Bioeng. v.75 Production of cellulose from glucose by Acetobacter xylinum Masaoka, S.;T. Ohe;N. Sakota
  13. Polymer Degradation and Stability v.59 Improved production of bacterial cellulose and its application potential Vandamme, E. J.;S. De Baets;A. Vanbaelen;K. Joris;P. De Wulf
  14. Biosci. Biotech. Biochem. v.59 Production of cellulose from D-mannitol by Acetobacter xylinum KU-1 Oikawa, T.;T. Ohtori;M. Ameyama
  15. Nature v.179 Role of hexose phosphate in synthesis of cellulose by Acetobacter xylinum Schramm, M.;Z. Gromet;S. Hestrin
  16. Carbohydrate Research v.149 Control of cellulose synthesis in Acetobacter xylinum. A unique guanyl oligonucleotide is the immediate activator of the cellulose synthase Ross, P.;Y. Aloni;H. Weinhouse;D. Michaeli;P. Weinberger-Ohana;R. Mayer;M. Benziman
  17. Biosci. Biotech. Biochem. v.60 A synthetic medium for bacterial cellulose production by Acetobacter xylinum subsp. sucrofermentans Matsuoka, M.;T. Tsuchida;K. Matsushita;O. Adachi;F. Yoshinaga
  18. J. Gen. Microbiol. v.21 Cellulose production by Acetobacter acetigenum in defined medium Dudman, W. F.
  19. British Polymer J. v.22 Effects of culture conditions of acetic acid bacteria on cellulose biosynthesis Kamide, K.;Y. Matsuda;H. Iijima;K. Okajima
  20. Appl. Biochem. Biotech. v.63/65 Acetobacter cellulosic biofilms search for new modulators of cellulogenesis and native membrane treatments Fontana, J. D.;C.G. Joerke;M. Baron;M. Maraschin;A. G. Ferrira;I. Torriani;A. M. Souza;M. B. Soares;M. A. Fontana;M. F.Guimaraes
  21. Biosci. Biotech. Biochem v.58 Detection of carboxymethyl cellulase activity in Acetobacter xylinum KU-1 Oikawa, T.;M. Takagi;M. Ameyama
  22. Food Hydrocolloids v.6 Bacterial cellulose I. Two-stage fermentation process for cellulose production by Acetobacter aceti Okiyama, A.;H. Shirae;H. Kano;S. Yamanaka