• Journal of Microbiology and Biotechnology
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• 제14권4호
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• pp.707-714
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• 2004

How to improve the cell culture method on scaffolds is important in the tissue engineering fileld. In this study, we optimized seeding and culture methods to vascular smooth muscle cells (VSMCs) on biodegradable polymer scaffold. The primary culture of VSMCs obtained from canine external jugular vein was accomplished by applying the explant-derived method. The primary cultured VSMCs were seeded into scaffolds and then cultured by using various different methods; static or dynamic seeding, static or dynamic culture. The difference in proliferative response of VSMCs was analyzed with an alamar blue assay. Cell-polymer construct was examined by histochemical method and scanning electron microscopy. Mesh type scaffold ($10 \times 10 \times0.4 mm$) was made of polyglycolic acid (PGA) suture thread. The PGA mesh type scaffold was 45% in porosity, and 0.03 g in weight. The primary cultured VSMCs were confirmed with immunohistochemical staining using monoclonal anti-$\alpha$-smooth muscle actin. The density and distribution of proliferated VSMCs within the scaffold and cellular adherence on the surface of the scaffold showed better results in the static seeding condition than in the dynamic condition. Under the same condition of seeding method as the static condition, the dynamic culture condition showed enhanced proliferation rates of the VSMCs when compared to the static culture condition. In conclusion, to improve the VSMCs proliferation in vitro, static seeding is better than the dynamic condition. In the culture condition, however, culture under the dynamic status is better than the static condition. This was a pilot study to manufacture artificial vascular vessel by tissue engineering.

• 한국식품저장유통학회지
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• 제19권2호
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• pp.301-307
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• 2012

정치배양 현미식초(A)와 시판 국내산 현미식초 3종(B, C, D) 및 일본산 현미식초 3종(E, F, G)의 품질특성을 비교 분석하였다. 그 결과, 적정산도는 (A)가 6.39%로 가장 높게 나타났고 시판 국내산 현미식초가 4.52~6.32%, 일본산 현미식초가 4.51~4.89%로 나타났다. pH는 (A)가 3.28로 가장 높게 나타났고 시판 국내산 현미식초가 2.58~2.97, 일본산 현미식초가 3.03~3.27로 나타나 (A)의 pH는 일본산 현미식초와 비슷한 수치를 나타내었다. TN값을 측정한 결과, (A)는 0.24로 가장 높았고 시판 국내산 현미식초는 0.03~0.16로 나타나 제품간의 함량차이가 많았으나, 일본산 현미식초는 0.12~0.17로 비슷한 함량을 나타내었다. 유리당은 (A) 및 일본산 현미식초에서는 glucose만 소량 검출되었거나 불검출 되어 완전발효 되었고 시판 국내산 현미식초에서는 glucose 및 maltose 등 잔당함량이 높았다. 유기산 함량에서도 (A)와 일본산 현미식초가 유사한 경향으로 검출되었다. 따라서 정치배양 현미식초(A)는 총산함량, TN값 및 관능적 특성이 우수하며 시판 국내산 및 일본산 현미식초들과 비교하였을 때도 품질면에서 우수한 것으로 나타났다.

• International Journal of Advanced Culture Technology
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• 제5권2호
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• pp.63-73
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• 2017

In this paper, we propose algorithm for determining the static and dynamic posture using the acceleration sensor of smartphone. The measured acceleration values are then analyzed according to a preprocessing to the respective axis (X, Y, Z) and posture (standing, sitting, lying) presents static posture determination criterion. The proposed static posture determination condition is used for static posture determination and dynamic posture determination. The dynamic posture is determined by using regression linear equations. In addition, transition state can be grasped by SVM change in dynamic posture determination. Experimental results are presented using data and app. Experiments were performed using data collected from 10 adults.

• Clinical and Experimental Reproductive Medicine
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• 제43권3호
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• pp.146-151
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• 2016

Objective: This study was conducted to compare the effects of static culture, dynamic culture, and the combination of dynamic culture with specialized surfaces involving co-culture on human embryonic development. Embryos cultured using conventional static culture (SC) techniques served as a control group. We compared dynamic culture using micro-vibration culture (MVC) and micro-vibration with co-culture (MCoC), in which autologous cumulus cells were used as a specialized surface. Methods: We conducted a chart review of patients who were treated between January 2011 and November 2014 in order to compare embryonic development rates and pregnancy rates among the groups. Zygotes were cultured in micro-droplets, and embryos were subsequently selected for transfer. Some surplus embryos were cryopreserved, and the others were cultured for blastocyst development. A micro-vibrator was set at the frequency of 42 Hz for duration of 5 seconds per 60 minutes to facilitate embryo development. Results: No significant differences among the groups were present in patient's characteristics. However, the clinical pregnancy rates were significantly higher in the MVC group and the MCoC group than in the SC group. No significant differences were found in the blastocyst development rate between the SC group and the MVC group, but the blastocyst development rate in the MCoC group was significantly higher than in the SC and MVC groups. Conclusion: The clinical pregnancy rate was significantly increased by the application of micro-vibration to the embryonic cultures of poor responders. The blastocyst development rate was significantly increased by the application of MCoC to surplus embryos.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권6호
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• pp.564-570
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• 2005

In the present study, the protective effects of Bcl-2 over-expression in a suspension culture (without any adaptation) and spent medium (low nutrient and high toxic metabolite conditions) were investigated. In the suspension culture without prior adaptation, the viability of the control cell line fall to 0% by day 7, whereas the Bcl-2 cell line had a viability of 65%. The difference in the viability and viable cell density between the Bcl-2 and control cell lines was more apparent in the suspension culture than the static culture, and became even more apparent on day 6. Fluorescence microscopic counting revealed that the major mechanism of cell death in the control cell line in both the static and suspension cultures was apoptosis. For the Bcl-2 cell lines, necrosis was the major mode of cell death in the static culture, but apoptosis became equally important in the suspension culture. When the NS0 6A1 cell line was cultured in spent medium taken from a 14 day batch culture, the control cell line almost completely lost its viability by day 5, whereas, the Bcl-2 still had a viability of 73%. The viable cell density and viability of the Bcl-2 cell line cultivated in fresh medium were 2.2 and 2.7 fold higher, respectively, than those of the control cultures. However, the viable cell density and viability of the Bcl-2 cultivated in the spent medium were 8.7 and 7.8 fold higher, respectively, than those of the control cultures. Most of the dead cells in the control cell line were apoptotic; whereas, the major cell death mechanisms in the Bcl-2 cell line were necrotic.

• 한국식품저장유통학회지
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• 제18권1호
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• pp.26-32
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• 2011

본 연구에서는 누룩 첨가량을 달리하여 현미탁주를 제조 하고 이를 이용한 현미식초[교반배양(A) 및 정치배양(S)]의 품질 특성을 조사하였다. 누룩 첨가량에 따른 알코올 함량 은 누룩 첨가량이 많을수록 높게 나타났으며 누룩 30% (w/w) 이상 첨가구에서는 약 13.5%로 큰 차이가 없었다. 이를 이용한 교반 및 정치배양 현미식초의 품질특성을 비교한 결과, 교반배양(A)에서 적정산도는 발효 종료 후 CVA에서 5.97%로 가장 높았으며, 정치배양(S)에서는 발효 16일째이후 누룩 첨가량이 많은 순으로 적정산도가 높게 나타났으며, CVS와 DVS는 큰 차이가 없는 것으로 나타났다. 현미 식초의 유리아미노산 함량을 비교한 결과, 교반 및 정치배양에서 총 유리아미노산함량은 누룩 첨가량이 많을수록 증가하였으며, 정치배양 현미식초가 교반배양 현미식초에 비하여 7~30 mg%정도 더 높았다. 특히, 현미의 주요한 기능성 강화물질로 알려진 ${\gamma}$-aminobutyric acid는 교반배양 현미식초보다 정치배양 현미식초에서 약 3~5배 더 높은 것으로 나타났다. 따라서 본 연구에서는 누룩 첨가량 및 초산발효 배양방법에 따라서 유리아미노산 함량 차이가 큰 것으로 판단되며, 정치배양의 경우 향후 숙성과정에서 변화를 거치면서 향기성분, 관능적 특성에 차이가 있을 것으로 예상되어 다양한 식초의 품질변화에 관한 연구가 요구된다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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• pp.350-351
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• 2000

We isolated cellulose-producing bacteria from persimmon vinegar(Korea traditional fermentation food). Some of these strains were selected for cellulose production in agitation culture. On the other hand, it was also found that strains suitable for static culture production were not necessarily suitable for agitation culture. Therefore we estimated the cellulose production of these isolates in static culture. To determine nutritional requirement for the production of bacterial cellulose, several nutrients as carbon source, nitrogen and mineral salt were tested.

• 한국식품과학회지
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• 제35권6호
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• pp.1150-1154
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• 2003

식초공장에서 Acetobacter pasteruianus로 정치배양중인 식초 생산과정과 숙성중인 현미식초를 오염시켜 균막을 형성한 것으로부터 균을 분리하여 식초를 오염시키는 균의 특성을 밝히고자 하였다. 공장에서 식초제품을 생산하거나 가정에서 식초제품을 사용할 경우에 오염을 일으키는 초산균의 생육에 관하여 배양조건 및 생육억제 방법을 검토하였다. 초산균에 오염되어 균막이 형성된 식초로부터 초산균을 분리하여 형태학적, 배양적 및 생리학적 특성을 검토한 결과 Acetobacter속에 포함됨을 알 수 있었다. 그리고 정치배양 기간 동안에 셀룰로오스 생산량을 확인하고자 14일간 배양한 결과 두꺼운 셀룰로오스를 생성하여 Acetobacter xylinum으로 판단되었으며 7%이상의 초산농도와 10%의 에탄올농도, 1.5%의 NaCl농도, $10^{\circ}C$ 이하와 $40^{\circ}C$ 이상의 온도에서 생육할 수 없었다.

• 한국식품영양과학회지
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• 제39권9호
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• pp.1366-1372
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• 2010

알코올 발효 효모 종류를 달리하여 현미탁주를 제조하고 이를 이용한 현미식초(정치배양 및 교반배양)의 품질 특성을 조사하였다. 효모 종류에 따른 알코올 함량은 B가 14.3%로 가장 높았으며 적정산도는 모든 구간에서 0.6% 이하로 나타났다. 이를 이용한 교반 및 정치배양 현미식초의 품질특성을 비교한 결과, 교반배양구간에서는 초기산도 1.0%, 초기 pH는 3.9~4.0에서 발효 7일째 DV구간에서 산도가 6.05%로 가장 높았고 AV, CV 및 BV가 5.64, 5.55 및 5.32% 순으로 나타났다. 또한 정치배양구간에서도 산도는 발효 14일째까지 꾸준히 증가하여 5.01~5.31%로 나타났고 발효 16일째부터 조금씩 감소하는 경향이었다. 효모 종류에 따른 현미식초의 산도 및 pH 차이는 크게 나타나지 않았다. 현미식초의 유리아미노산 함량을 비교한 결과, 교반배양구간에서 총 유리아미노산 함량은 BV, DV, AV 및 CV 순으로 높게 나타났고 필수 유리아미노산 함량은 BV에서 100 mg% 이상으로 가장 높았다. 정치배양구간에서 유리아미노산 함량은 모든 구간에서 교반배양구간보다 높은 함량을 나타내었으며 serine, alanine, valine, isoleucine, leucine 및 $\gamma$-aminobutyric acid 등이 교반배양구간에 비하여 높았으며 특히 $\gamma$-aminobutyric acid는 45.69~52.27 mg%로 약 10배 이상 높게 나타났다. 현재까지의 결과는 현미식초의 품질특성은 효모 종류보다는 초산발효 방법에 따른 품질 차이가 더 큰 것으로 나타났다. 그러나 향후 숙성과정에서 변화를 거치면서 향기성분, 관능적 특성에 차이가 있을 것으로 예상되어 다양한 식초의 품질인자에 관한 연구가 요구된다.

• International Journal of Advanced Culture Technology
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• 제4권3호
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• pp.56-61
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• 2016

Central motion techniques are to mention the central-motion by the limpness motion function and limpness sensory unit function on the body. Central body motion is consisted of the limpness central function by the central body system. To evaluate the signal of central body motion, we are investigated a limpness value of the central function by the central body function on the static state. The concept of limpness motion function was checked the reference of limpness motion signal and limpness sensory signal by the central motion body. For assessment on the limpness sensory variation of the maximum and average in terms of central motion from the static function, and limpness value that was a limpness value of the vision condition of the Vi-${\lambda}_{MAX-AVG}$ with $8.71{\pm}-3.2units$, that was a limpness value of the vestibular condition of the Ve-${\lambda}_{MAX-AVG}$ with $3.05{\pm}-6.52units$, that was a limpness value of the somatosensory condition of the So-${\lambda}_{MAX-AVG}$ with $2.4{\pm}1.9units$. The static sensory motion was made mention of check out at the condition of the limpness sensory unit motion for the comparable values of limpness central motion that was expressed the analysis capacity by the limpness nerve system. Limpness sensory system will be to propose of the minute motion by static central motion situation and was to imply a limpness motion data of static body sensory function.