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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Characterization of a pH/Temperature-Sensitive Hydrogel Synthesized at Different pH and Temperature Conditions

pH/온도-동시 민감성 Hydrogel의 합성조건에 따른 특성 연구

  • Published : 2000.12.01
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Abstract

A hydrogel, poly(N-isopropylacrylamide-co-N, N-dimethylaminopropylmethacrylamide), sensitive to both pH and temperature, was synthesized and characterized at $^13∼23{\circ}C$ and pH of 10.3∼12.3. The gel was more transparent and mechanically stronger at lower preparation temperature and pH. Large pores observed in scanning electron microscope seem to be responsible for the lower biomolecular separation efficiency. The lower critical solution temperature (LCST) decreased at a higher polymerization temperature. At $25^{\circ}C$, which is lower than the LCST, the gel was swollen regardless of the solution pH. At $40^{\circ}C$, however, the gel was swollen at neutral and acidic pHs even though the temperature was higher than the LCST. The gel collapse pH, defined as the point at which the gel made its largest volume decrease per unit pH increment, increased as the gel preparation temperature increased.

온도 및 pH에 동시에 민감한 하이드로젤 poly(N-isopropy­l lacrylamide-co-N,N-dimethylaminopropy Imethacrylamide)을 온도 (13, 15.5, 18, 20.5 및 $23^{\circ}C$)와 pH (10.3, 11.3 및 12.3)를 달리 하여 합성하고 이 젤들의 외형, 기계적 강도, 젤 표면모양, LCST, 수축 pH 및 젤의 팽윤 특성을 연구하였다. 합성온도 및 합성 pH가 낮을수록 젤의 외형은 투명하였 고 기계적 강도는 높았다. SEM 관찰 결과 단백질 보다 더 큰 pore들 때문에 분리효율이 감소되는 것으로 사료된다. 합 성온도나 합성 pH의 증가는 LCST를 낮추었다. 외부온도가 LCST보다 낮은 $25^{\circ}C$ 에 서는 모든 합성온도와 합성 pH에 대 하여 젤은 전 pH에 걸쳐 팽윤된 상태에 있었다. $40^{\circ}C$에서는 LCST보다 높은 온도임에도 불구하고 poly (NIPAAm-co­D DMAPMAAm) 하이드로젤은 pH가 중성 및 산성 영역에서 팽윤되었다. 합성온도가 증가함에 따라 젤 부피가 가장 큰 폭으로 변하는 수축 pH가 더 높아졌다.

Keywords

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