Biotechnology and Bioprocess Engineering:BBE

  • 제5권3호
  • /
  • Pages.191-195
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  • 2000
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  • 1226-8372(pISSN)
  • /
  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

High-Performance liquid Chrmatogrphic and Tandem Mass Spectrometric Quantitation of N7-Methyldeoxyguanosin in Methylated Calf Thymus DNA

  • Chae, Whi-Gun (Department of Biochemistry, School of medicien, Gatholic University of Taegu, Taegu 705-034)
  • 발행 : 2000.06.01
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초록

Quantitation of N7-methyldeoxyguanosine (N7-MedG) produced in the in vitro N-methly-N-nitrosuourea (NMU) action on thymus DNA has been achieved by enzymatic degradation, liquid chromatoraphic separaphic separation and desorption chemical ionization tandem mass spectrometry. In conjunction with the resolving power HPLC in the separation of isomers, desorprion chemical ionization tandem mass spectrometry has utilized in determining modified nucleosides at low levels using a stable-isotope labled compound as an internal reforence. The quantitative estimation of N7-methyldeoxyguanosine was previously established by an independent HPLC analysis of methylated calf thymus DNA. A sensitive and specific methodogy for the quantitation of N7-MedG at the picomole level using HPLC combined with tandem mass spectrometry without radioisotope labeling process is presented. The potential of the liquid chromatoraphic tandem mass exposure to methlation agents in vitro.

키워드

참고문헌

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