생명과학회지 (Journal of Life Science)

  • 제10권2호
  • /
  • Pages.115-124
  • /
  • 2000
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지

복어간 폐기물로부터 Tetrodotoxin, DHA 및 EPA의 분리

Separation of Tetrodotoxin, DHA and EPA from Pufferfish Liver Waste

  • 차병윤 (경성대학교 공과대학 식품공학과) ;
  • 최진석 (경성대학교 공과대학 식품공학과) ;
  • 임정규 (경성대학교 공과대학 식품공학과) ;
  • 이동익 (경성대학교 공과대학 식품공학과) ;
  • 이원갑 (경성대학교 공과대학 식품공학과) ;
  • 이은열 (경성대학교 공과대학 식품공학과) ;
  • 김희숙 (경성대학교 공과대학 식품공학과) ;
  • 김동수 (경성대학교 공과대학 식품공학과)
  • 발행 : 2000.04.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The present study was undertaken to separate the available components effectively, such as tetrodotoxin(TTX), docosahexaenoic acid(DHA, C22:6,ω-3) and eicosapentaenoic acid (EPA, C20:5,ω -3) from pufferfish liver waste, which are known to have high values as bioactive materials. By using ultrafiltration, it was possible to separate high contents of 68mg TTX from pufferfish liver waste. In contrast, by activated charcoal column, it was to obtain about 54mg TTX. The recovering ratios were 65.3% and 45.0% in the two different methods of ultrafiltration and activated charcoal column, respectively. From the results of HPLC and gas chromatography-mass spectrometry(GC-MS), the obtained toxins were identified to be TTX and its derivatives. In addition, it was also possible to obtain 72.3g DHA and 11.4g EPA from 1kg of pufferfish liver by high performance liquid chromatography (HPLC). These amounts of DHA and EPA were also 17.70% and 1.04% in the total lipid of pufferfish liver oil from analysis of gas chromatography(GC), respectively.

키워드

참고문헌

  1. Engalnd, Fishing News Books Advances in fish science and technology Ackman, R. G.;J. Connel
  2. Am. J. Clin. Nutr. v.33 The composition of the Eskimo food in North western Greenland Bang, H. O.;J. Dyerberg;H. M. Sinclair
  3. Can. J. Biochem. Physiol. v.37 A rapid method of total lipidextraction and purification Bligh, E. G.;W. J. Dyer
  4. J. Food Hyg. Soc. Japan v.29 Determination of tetrodotoxin in pufferfish and shellfish by high performance liquid chromatography Funchi, Y.;S. Morisaki;T. Nagata;K. Shimazaki;T. Noguchi;N. Ohtomo;K. Hashimoto
  5. Am. J. Clin. Nutr. v.107 Fish oil consumption and decreased risk of cardiovascular disease: A comparison of finding from animal and human feeding trial Herold, P. M.;J. E. Kinsella
  6. Biophys. J. v.15 The receptor for tetrodotoxin and saxitoxin Hille, B.
  7. Nippon suisan Gakkaishi v.54 Toxicological studies on several species of puffers in Taiwan Hwang, D. F.;T. Noguchi;O. Arakawa;T. Abe;K. Hashimoto
  8. Korea Bull. Korean Fish Soc. v.27 Toxicity of several puffers collected at a fish market of Pusan Jeong, D. Y.;D. S. Kim;M. J. Lee;S. R. Kim, D. S. Byun;H. D. Kim;Y. H. Park
  9. J. Korean Food and Nutr. v.9 Anatomical Toxicity of Pufferfishes,Chinese Fugu obscurus and Indonesian Lagocephalus wheeleri Kim, D. S.;H. J. Lee;M. J. Lee;H. D. Kim
  10. J. Korean Fish Soc. v.28 toxicity of the imported pufferfish, Fugu flavidus ("Samchaebog"), from china Kim, D. S.;S. R. Kim;M. J. Lee;M. H. Seol;D. Y. Jeong;H. D. Kim
  11. J. Korean Fish Soc. v.28 Toxicity of the Pufferfish Fugu stictonutus ("Ggachilbog") Collected at a Fish Market of Pusan Kim, K. C.;J. W. Park;M. J. Lee;S. R. Kim;D. S. Kim;H. D. Kim;Y. H. Park
  12. Seafoods and fish oil in human health and disease Kinsella, J. E.
  13. Bull. Korean Fish Soc. v.21 Utilization of Polyunsaturated Lipids in red Muscled Fishes, 3. The Conditions of Refining, Decoloring, and Deodorization for Processing of Refined Sardine Oil Lee, K. H.;I. H. Jeong;J. S. Suh;J. H. Ryuk
  14. Analytical Chemistry v.38 Rapid preparation of fatty acid esters from lipids for gas chromatographic analysis Metcalfe, I. D.;A. A. Schmitz;J. R. Pelka
  15. Shokuhin Eisei Kensa Shishin v.2 Ministry of Health and Welfare
  16. Nippon Suisan Gakaishi v.53 Analysis of paralytic shellfish poison and tetrodotoxin by ion-pairing high performance liquid chromatography Nagashima, Y.;J. Maruyama;T. Noguchi;K. Hashimoto
  17. Nippon Suisan Gakaishi v.59 Determination of EPA and DHA Content in Extracted Fish Oil by Modified HPLC Nakajima, S.;I. Matsushita;T. Tsuchiya
  18. Lipids v.2 Quantitative analysis of brain ad spinach leaf lipids employing silicic acid column chromatography and acetone for elution of glycolipids Rouser, G.;G. Kritchevsky;G. Siomon
  19. Arch. Toxicol. v.44 Detection of tetrodotoxin in autopsy materials by gas chromatography Suenaga, K.;S. Kotoku
  20. INFORM. v.6 Microbial production of long chain PUFAs Ward, O. P.