Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Implications of Exonuclease Activity of Bacteriophage P2 Old Protein for Lambda Exclusion

  • Kim, Kwang-Ho (Department of Bioscience and Biotechnology, Hankik University of Foreign Studies) ;
  • Park, Chan-Hee (Department of Bioscience and Biotechnology, Hankik University of Foreign Studies) ;
  • Yeo, Hyeon-Joo (Department of Bioscience and Biotechnology, Hankik University of Foreign Studies) ;
  • Kee, Young-Hoon (Department of Bioscience and Biotechnology, Hankik University of Foreign Studies) ;
  • Park, Jung-Chan (Department of Bioscience and Biotechnology, Hankik University of Foreign Studies) ;
  • Myung, Hee-Joon (hjmuung@maincc.hufs.ac.kr)
  • Published : 2000.04.01
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Abstract

Temperate bacteriophage P2 has a nonessential gene called old(overcoming lysoginization defection). In the presence of old, the growth of the host (Escherichia coli) with recBC- genotype is ingibited, and another bacteriophage, lambda, cannot superinfect. The Old protein has been shown to possess an exonuclease actibity. Three mutant P2s(old 1, old 17, old 49) which did gene was coned into expression vectors to produce hexahistidine-tagged proteins. The proteins were affinity-purified and shown to lose its exonuclease activity on both double-stranded and single-stranded DNA substrates. Thus, it was concluded that the lambda exclusion was related to Old's exonuclease activity.

Keywords

References

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