Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Chemosystematic and Phenotypic Characterization of Gram-positive Bacteria from Coastal Seawater, Korea

한국 근해에서 분리한 그람양성 세균의 화학 분류학적 및 표현형적 특성

  • Published : 2000.09.01
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Abstract

Twenty-five halotolerant gram-positlve bacteria were isolated from the coastal seawater 01 Cheju Island and Incheon J&yakdo Chemosystematic and phenotypic characteristics were used to iuvestigate the taxonomic position of these bacteria. According to their chemosystematic characteristics, the twenty-tive isolates were divided into 4 groups. Group 1 bacteria possesed 40.1 to 49.9 inol% m DNA G+C content, menaquinone-7 as a major quinone, and meso-Alpm as a diamino acid of peptidoglycan. Group 1 tam were identified as Bacilluspumilis, Bacillus lichenifbrrizis, Bacillus megaterium, Bncill~rsubtilis. Group 2 bacteria possessed 63.9 to 66.4 mol% and MK-8. They were all in the genus Arth~obaclm-. Group 3 bacteria possessed 31.0 to 37.6 mol% and MK-7. They were identified as Staphylococcus haeniol.viicvs, Siaph~~lococc~is sapropl~j~ticns, and Siaphylococcus intermediru.. Group 4 bacterium possessed 72.0mol% and MK-8 and was identified as ~Lficrococcus ltdtezm. Bacillus species accounted for 68% of h e total isolates.

제주도의 여섯 지역과 인천 작약도 근해의 해수로부터 내염성 그람양성 세균 25균주를 분리하여 화학 분류학적 특징과 표현형적 특성을 조사하였다. 분리된 균주들은 화학 분류학적 특징에 의거하여 4개의 group으로 구분되었다. Group1은 40.~49.9 mol% 의 G+C함량과 MK-7의 menaquinome type, peptidoglycan의 주요 아미노산으로 meso-A2pm을 함유하며 Bacillus pumilus와 Bacillus licheniformis, Bacillu megaterium, bacillus subtilis로 동정되었으며, Group 2는 63.9~66.4 mol% 의 G+C 함량과 MK-8을 함유하는 Arthrobacter속 세균이었으며 , Group 3은 31.0~37.6 mol%의 G+G 함량과 MK-7을 함유하며 Staphylococcus haemolyticus, Staphylococcus saprophyticyus, Staphylococcus intermedius 로 , Group 4는 72.0 mol% 의 G+C 함량과 MK-8을 주요 quinone으로 함유하는 Micrococcus luteus로 동정되었다. 분리된 세균들 중 Bacillus 속 세균들은 68%로 우세한 분포를 나타내었다.

Keywords

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