한국어병학회지 (Journal of fish pathology)

  • 제13권1호
  • /
  • Pages.7-13
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  • 2000
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  • 1226-0819(pISSN)
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  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
권호 표지

양식새우(Penaeus chinensis)에서의 White Spot Baculovirus의 분리 및 특성

Isolation and Characterization of White Spot Syndrome Baculovirus in Cultured Penaeid Shrimp (Penaeus chinensis)

  • Heo, M.S. (Faculty of Applied Marine Science, Cheju National University) ;
  • Sohn, S.G. (National Fiseries Research & Development Institute) ;
  • Sim, D.S. (National Fiseries Research & Development Institute) ;
  • Kim, J.W. (National Fiseries Research & Development Institute) ;
  • Park, M.A. (National Fiseries Research & Development Institute) ;
  • Lee, J.S. (National Fiseries Research & Development Institute) ;
  • Choi, D.L. (National Fiseries Research & Development Institute) ;
  • Jung, S.H. (National Fiseries Research & Development Institute) ;
  • Kim, Y.J. (Department Fish Pathology, Yosu National University) ;
  • Oh, M.J. (Department Fish Pathology, Yosu National University)
  • 발행 : 2000.05.30
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초록

1993년부터 한국 서해안의 새우 양식장에서는 양식새우의 대량폐사가 일어났다. 외부증상은 두흉갑과 표피에 흰 반점이 나타났고 어류주화세포에는 배양되지 않았다. 열($50^{\circ}C$) 및 강산(pH 3)에는 쉽게 실활되었으나 강알카리(pH 11)에는 내성이 강했다. 바이러스의 입자 형태는 Rod Shaped한 형태를 보였다. 바이러스 단백질의 분석결과는 Hypodermal Hematopoietic Necrosis Baculovirus(HHNBV)와 유사했고 바이러스 핵산분석 결과는 약 114kb로 Penaeid Acute Viremia(PAV)와 유사했다.

Beginning in the summer of 1993, a serious mortality among cultured penaeid shrimp occurred in the western sea of Korea. The typical sign of this disease was white spots inside the surface of the carapace. Cytopathic effect (CPE) were not observed by virus in CHSE-214, RTG-2, but not by pH 11. A nonoccluded rod-shaped form virus was observed by electron microscopy in the lymphoid organ. The virion was bacilliform virus and sourrounded by a virion envelope. Its virion protein was found to be similar to hypodermal and hematopoietic necrosis virus (HHNBV) by analysis of virion proteins in SDS-PAGE. The genome of virus is double stranded DNA molecule whose full length was about 114kb. It was similar to penaeus acute viremia (PAV) of Japan.

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