Journal of Society of Preventive Korean Medicine (대한예방한의학회지)

Society of Preventive Korean Medicine (대한예방한의학회)
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Studies on the cytotoxicity and Antimicrobial Effects of the Extract of Houttuynia cordata (IV)

어성초 추출물의 세포독성과 향균효과 (IV)

  • Lee Jeong-Ho (Department of Natural Products, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Park Nang-Kyu (Department of Natural Products, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Yang Eun-Yeong (Kunhil Pharmaceutical Co., LTD.) ;
  • Lee Hyun-Ok (Department of Hygiene, Wonkwang Health College) ;
  • Han Dong-Min (Division of Chemistry Technology and Biological Science, College of Natural Sciences, Wonkwang University) ;
  • Baek Seung-Hwa (Department of Natural Products, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 이정호 (원광대학교 한의학전문대학원 천연물학교실) ;
  • 박낭규 (원광대학교 한의학전문대학원 천연물학교실) ;
  • 양은영 (건일제약(주) 중앙연구소) ;
  • 이현옥 (원광보건대학 치위생과) ;
  • 한동민 (원광대학교 자연과학대학 화학기술.생명과학부) ;
  • 백승화 (원광대학교 한의학전문대학원 천연물학교실)
  • Published : 2000.11.29
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Abstract

This study was carried out to evaluate cytotoxic effects of Houttuynia cordata Thunberg extracts on murine leukemia tumor cell lines. Disruptions in cell organelles were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazol iumbromide (MMT) assay. The comparison of $IC_{50}$ values of Houttuynia cordata Thunberg extracts on $L1210,\;P388D_1$ and Vero cell lines showed that the methanol extract of Houttuynia cordata Thunberg indicated the most antitumor activity in the MTT assay. In order to develop a antimicrobial agent, dried Houttuynia cordata Thunberg was extracted with several solvents, and then antimicrobial activity was investigated. The minimal inhibitory concentration (MIC) of the extracted substance against microorganisms were also examined. Antimicrobial activity of amocla and ketoconazole as references was compared to those of other solvent extracts such as $H_2O$, n-hexane, chloroform, ethyl acetate ethanol and methanol. The antimicrobial activity of all extracts from the sample had growth inhibition activity against gram-negative bacteria, yam-positive bacteria and fungi $(MIC,\;>\;200\;{\mu} g/ml)$. These results suggest that the methanol soluble extract of Houttuynia cordata Thunberg may be a valuable choice for the studies on the treaeent of murine leukemia tumor cell lines and antimicrobial agents.

Keywords

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