Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Studies on the Cytotoxicity and Antimicrobial Effects of the Extract of Ajuga multiflora Bunge

조개나물 추출물의 세포독성과 항균효과

  • Ryu, Myeng-Hwan (Department of Herbalogy, School of Oriental Medicine, Wonkwang University) ;
  • Aeam, Yong-Dea (Department of Herbalogy, School of Oriental Medicine, Wonkwang University) ;
  • Byun, Jong-Ho (Department of Herbalogy, School of Oriental Medicine, Wonkwang University) ;
  • Cho, Hoon (Kunkil Pharmaceutical Co., Ltd.) ;
  • Yang, Eun-Yeong (Kunkil Pharmaceutical Co., Ltd.) ;
  • Kang, Kil-Ung (Department of Natural Products, Professional Graduate School of Oriental Medicine Wonkwang University) ;
  • Shin, Min-Kyo (Department of Herbalogy, School of Oriental Medicine, Wonkwang University) ;
  • Baek, Seung-Hwa (Department of Natural Products, Professional Graduate School of Oriental Medicine Wonkwang University)
  • 류명환 (원광대학교 한의과대학 본초학교실) ;
  • 엄용대 (원광대학교 한의과대학 본초학교실) ;
  • 변종호 (원광대학교 한의과대학 본초학교실) ;
  • 조훈 (건일제약(주) 중앙연구소) ;
  • 양은영 (건일제약(주) 중앙연구소) ;
  • 강길웅 (원광대학교 한의학전문대학원 천연물학교실) ;
  • 신민교 (원광대학교 한의과대학 본초학교실) ;
  • 백승화 (원광대학교 한의학전문대학원 천연물학교실)
  • Published : 2000.03.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out to evaluate cytotoxic effects of Ajuga multiflora Bunge extracts on murine leukemia tumor $(P388D_1)$ cell lines. Disruptions in cell organelles were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) assay. The comparison of $IC_{50}$ values of Ajuga multiflora Bunge extracts in L1210 and $P388D_1$ cell lines showed that their susceptibility to these extracts decreased in the following order: Adriamycin>methanol extract>chloroform extract>ethyl acetate extract>hexane extract>water extract by the MTT assay. In order to develop an antimicrobial agent, dried Ajuga multiflora Bunge was extracted with several solvents, and then antimicrobial activity was investigated. The minimal inhibitory concentration (MIC) of the extract against microorganisms were also examined. Antimicrobial activities of amocla and ketoconazole as references were compared to those of extracts of $H_2O$, n-hexane, chloroform, ethyl acetate and methanol. The antimicrobial activity of all extracts from the sample had growth inhibition activity against gram-negative bacteria, gram-positive bacteria and fungi $(MIC>200\;{\mu}g/ml)$. These results suggest that the methanol soluble extract of Ajuga multiflora Bunge may be a valuable choice for the studies on the treatment of murine leukemia tumor cell lines.

Keywords