Clinical and Experimental Reproductive Medicine
- Volume 26 Issue 3
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- Pages.399-405
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- 1999
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- 2233-8233(pISSN)
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- 2233-8241(eISSN)
The Effects of Oocyte Preparation on the Developing Capacity of Human Oocytes at Intracytoplasmic Sperm Injection (ICSI)
난자내 정자 직접주입술에서 난자의 처리방법이 난자의 발생능력에 미치는 영향
- Park, Kee-Sang (Department of Obstetrics and Gynecology, School of Medicine, Kyungpook National University) ;
- Lee, Taek-Hoo (Department of Obstetrics and Gynecology, School of Medicine, Kyungpook National University) ;
- Song, Hai-Bum (Department of Animal Science, Taegu University) ;
- Chun, Sang-Sik (Department of Obstetrics and Gynecology, School of Medicine, Kyungpook National University)
- 박기상 (경북대학교 의과대학 산부인과학교실) ;
- 이택후 (경북대학교 의과대학 산부인과학교실) ;
- 송해범 (대구대학교 자연자원대학 축산학과) ;
- 전상식 (경북대학교 의과대학 산부인과학교실)
- Published : 1999.09.30
Abstract
Objective: In the preparation of ICSI, cumulus and corona cells should be removed from the oocytes by using a combination of enzymatic (hyaluronidase) and mechanical (pipetting) methods. But little is known about the effects of different degrees of oocyte denudation and incubation time between denudation and sperm injection on the outcomes of ICSI. The aim of this study was to evaluate the effects of varying the degrees of oocyte denudation and the lengths of incubation time from denudation to sperm injection on the outcomes of ICSI. Methods: In experiment 1, patients (oocytes) were grouped into group A and B according to the degree of denudation, complete and partial, respectively. In experiment 2, patients (oocytes) were grouped into group I, II and III according to the length of incubation time of denuded oocytes until sperm injection as < 1,