Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Modification of Acetobacter xylinum Bacterial Cellulose Using Dextransucrase and Alternansucrase

  • Kim, Do-Man (Department of Biochemical Engineering, Chonnam National University, The Research Institute for Catalysis, Research Center for New Biomaterials in Agriculture, Seoul National University) ;
  • Kim, Young-Min (Department of Biomedical Engineering, Chonnam National University) ;
  • Park, Mi-Ran (Department of Biomedical Engineering, Chonnam National University) ;
  • Park, Don-Hee (Department of Biochemical Engineering, Chonnam National University)
  • Published : 1999.12.01
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Abstract

In addition to catalyzing the synthesis of glucan from sucrose as a primary reaction, glucansucrase also catalyzes the transfer of glucose from sucrose to other carbohydrates that are present or are added to the reaction digest. Using dextransucrase and altemansucrase, prepared from Leuconostoc mesenteroides B-742CBM and B-1355C, respectively, we modified the bacterial cellulose in Acetobacter xylinum ATCC10821 culture, and then produced a characteristic cellulose that is soluble and has a new structure. There were also some partially modified insoluble cellulose and oligosaccharides in the modification culture. After methylation and following acid hydrolysis of both the soluble and insoluble glucans, there were ($1{\rightarrow}4$) as well as ($1{\rightarrow}6$) and ($1{\rightarrow}3$) glycosidic linkages in the soluble glucan.

Keywords

References

  1. J. Chem. Soc. v.49 An acetic ferment which forms cellulose Brown, A. J.
  2. Prep. Biochem. v.20 A facile purification of Leuconostoc Mesenteroides B-512FM dextransucrase Fu, D.;J. F. Robyt
  3. Arch. Biochem. Biophy. v.283 Acceptor reactions of maltodextrins with Leuconostoc mesenteroides B-512FM dextraansucrase Fu, D.;J. F. Robyt
  4. J. Biochem. v.56 Synthesis of cellulose by Acetobacter xylinum: Micromethods for the determination of cellulose Hestrin, S.;M. Schramm
  5. Carbohyd. Res v.74 The α-D-glucopyanosidic linkages of dextrans: Comparison of percentages from structural analysis by periodate oxidation and by methylation Jeanes, A.;F. R. Seymour
  6. Ann. RCNBMA v.4 Characteristics of dextransucrase of Leuconostoc mesenteroides B-1299 mutants, and enzymatic modification of the amylose and pullulan Kim, D.;J.-S. Baek;J.-H. Lee
  7. Enzyme Microb. Tech. v.16 Production and selection of mutants of Leuconostoc mesenteroides constitutive for glucansucrases Kim, D.;J. F. Robyt
  8. Enzyme Microb. Tech. v.17 Production, selection, and characteristics of mutants of Leuconostoc mesenteroides B-742 constitutive for dextransucrase Kim, D.;J. F. Robyt
  9. Enzyme Microb. Tech. v.17 Dextransucrase constitutive mutants of Leuconostoc mesenteroides NRRL B-1299 Kim, D.;J. F. Robyt
  10. J. Microbiol. Biotechnol. v.9 Enzymatic modification of cellulose using Leuconostoc mesenteroides B-742CBM dextransucrase Kim, D.;Y.-M. Kim;M-R. Park;H.-J. Ryu;D.-H. Park;J. F. Robyt
  11. Kor. J. Appl. Micorbiol. Biotechnol. v.26 Modification of pullulan using dextransucrase and characterization of the modified pullulan Lee, J.-H.;D. Kim;H.-J. Ryu;S.-J. Heo;D.-Y. Jhon;N.-S. Han;J. F. Robyt
  12. Kor. J. Appl. Microbiol. Biotechnol. v.26 Modification of starch using dextransucrase and characterization of the modified starch Lee, J.-H.;D. Kim;J.-S. Baek;K.-H. Park;N.-S. Han;J. F. Robyt
  13. Carbohydr. Res. v.292 Simplified and improved methylation analysis of saccharides, using a modified procedure and thin-layer chromatography Mukerjea, R.;D. Kim;J. F. Robyt
  14. U.S. Patent No. 3,133,856 Process for modifying polysaccharides and products thereof Neely, W. B.
  15. Food Technol. v.51 High technology: Taking ingredients to a new level Pszezola, D. E.
  16. Arch. Biochem. Biophys. v.174 The mechanism of dextransucrase: Biosynthesis of branch linkages by acceptor reactions with dextran Robyt, J. F.;H. Taniguchi
  17. Carbohyd. Res. v.121 Relative, quantitative effects of acceptors in the reaction of Leuconostoc mesenteroides B-512F dextransucrase Robyt, J. F.;S. H. Eklund