Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Development of Apoptosis Model and Bioimmune Responses and Morphological Characterization in Experimental Animal II. Activities of Serum Hepatic Enzyme and Histological Findings between Apoptosis and Hepatic Tumorigenesis

실험동물에서 apoptosis의 모델개발과 생체면역반응 및 형태학적 특징 II. Apoptosis 및 hepatic tumorigenesis 과정에서의 혈청 간 효소활성치 및 조직소견

  • 강정부 (경상대학교 수의과대학(동물의학연구소)) ;
  • 하우송 (경상대학교 의과대학(암 연구소)) ;
  • 곽수동 (경상대학교 수의과대학(동물의학연구소)) ;
  • 김지경 (경상대학교 수의과대학(동물의학연구소))
  • Published : 1999.06.01
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Abstract

Hepatic tumorigenesis was induced by ad libitum feeding of diethylnitrosamine (DEN) only. We could also observe hepatic tumor induction in 100% of DEN treated rats without any other cocarcinogen. The liver specific enzyme activities (AST, ALT, ALP, ${\gamma}$-GTP) were significantly increased (P<0.05) in all treated groups compared to control and induced apoptosis groups. In histopathological analysis, the altered foci, hyperplastic nodules, neoplastic nodules, adenomas and carcinomas were observed in liver tumors induced by administration of DEN in rats. Lipopolysaccharide-induced apoptosis in D-galactosamine sensitized mice was investigated in hepatocytes in vivo. Typical morphological changes of apoptosis were detectable in liver 12 hr and 24 hr after the injection of Lipopolysaccharide (5 $\mu\textrm{g}$) and D-galactosamine (20 mg) to mice. It was suggested that organ specific enzyme activities and morphological findings might be very useful for understanding the role of hepatic tumorigenesis including the apoptotic cell death.

Keywords

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