식물조직배양학회지 (Korean Journal of Plant Tissue Culture)

한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지

액아배양에 의한 희귀 수종 미선나무의 기내번식

Micropropagation of a Rare Species, Abeliophyllum distichum Nakai. via axillary bud culture

  • 문흥규 (임업연구원 임목육종부 생물공학과) ;
  • 석진영 (임업연구원 임목육종부 생물공학과) ;
  • 권영진 (임업연구원 임목육종부 생물공학과) ;
  • 손성호 (임업연구원 임목육종부 생물공학과)
  • 발행 : 1999.03.01
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초록

미선나무의 기내번식을 위하여 MS배지에 세 가지 싸이토키닌 (zeatin, kinetin 및 BA)을 세 가지 농도로 처리하여 액아 배양을 실시하였다. 줄기유도는 BA가 가장 효과적이었고, 생장은 zeatin이 좋았다. Kinetin은 2.0 및 5.0 mg/L 처리에서 줄기발생 효과가 있었으나 그 효과는 zeatin, BA에 비해 저조했다. 500 lux정도의 약광 하에서 배양은 싸이토키닌 처리에 관계없이 줄기의 생장을 촉진하였다. 발근은 IBA가 첨가된 1/2 GD배지에서 주효했다. 발근된 개체는 인공배양토에서 100% 활착되었고, 정상 생장이 가능했다. 이상의 결과에서 미선나무의 기내 번식이 가능함을 보여 주었다.

Different kinds of cytokinins and auxins were tested for both shoot induction and rooting in a rare species, Abeliophyllum distichum Nakai. BA in WPM medium was the most effective in shoot induction, whereas zeatin seemed to be the most suitable for shoot elongation. Kinetin, at the concentration of 2.0~5.0 mg/L showed an effect in shoot induction, but the effect was inferior to BA and zeatin. Rapid shoot elongation could be achieved when the cultures were maintained on the diffuse light condition (below 500 lux) regardless of cytokinin treatments. For in vitro rooting, IBA was investigated as the best type of auxin tested when half strength GD medium was incorporated. The frequency of rooting using the plant growth regulator and medium just mentioned above was revealed as approximately 90%. In addition, the survival rate of rooted plantlets was almost 100% in an artificial soil mixture.

키워드

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