Journal of Sericultural and Entomological Science (한국잠사곤충학회지)

Korean Society of Sericultural Science (한국잠사학회)
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Effects of Mulberry Leaf Extract on Oxygen Radicals and Their Scavenger Enzymes in Serum of Rats

뽕잎추출물이 혈청중의 활성산소 및 제거효소에 미치는 영향

  • 최진호 (부경대학교 식품생명공학부 생화학교실) ;
  • 김대익 (부경대학교 식품생명공학부 생화학교실) ;
  • 박수현 (부경대학교 식품생명공학부 생화학교실) ;
  • 김동우 (부경대학교 식품생명공학부 생화학교실) ;
  • 이종수 (부경대학교 식품생명공학부 생화학교실) ;
  • 류강선 (농업과학기술원 잠사곤충부) ;
  • 이완주 (농업과학기술원 잠사곤충부)
  • Published : 1999.12.01
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Abstract

This study was designed to investigate the effects of mulberry leaf extract (MLE) on oxygen radicals and their scavenger enzymes in serum of rats. Sprague-Dawley (SD) male rats (160${\pm}$10g) were fed experimental diets (MLE-100 and MLE-300 groups) added 100 and 300mg/kg BW/day for 6weeks. Triglyceride (TG) levels were significantly inhibited (10% and 20%) in MLE-100and MLE-300 groups, but there were no significant differences in total, LDL-and HDL- cholesterol levels in both MLE-100 and MLE-300 groups. Hydroxyl radical ($.$OH) formations resulted in a marked decreases(20∼25%) in MLE-100 and MLE-300 groups compared with control group, while superoxide radical (O2.-)and hydrogen peroxide formations resulted in a considerable decreases(7∼10% and 5∼10%) in MLE-100 and MLE-300 groups compared with control group. Lipid peroxide (LPO)and oxidized protein(>C=O group) productions resulted in a significant decreases (10% and 6∼10%) in MLE-100 and MLE-300 groups compared with control group. Superoxide dismutase (SOD)and catalase (CAT) activities were remarkably increased (30% and 40∼55%) in MLE-100 and MLE-300 groups, but glutathione peroxidase (GSHPX) activities were significantly increased (10∼15%) in MLE-100 and MLE-300 groups compared with control group. These results suggest that anti-aging effect of mulberry leaf extract (MLE) may play a pivotal role in attenuating a various agerelated changes.

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References

  1. J. Biol. Chem. v.249 no.4 Enzyme catalyzed free radical reactions with nicotinamide adenine nucleotide Chan, P. C.;bielski, B. H. J.
  2. Kor. J. Biochem. v.23 no.1 ipid peroxidation, aging and food restriction Choi, J. H.
  3. FEBS. Lett. v.128 Formation of a thiobarbituric acid-reactie substance from deoxyribose in the persence of iron salts Halliwell, B.;Gutteridge, J. M. C.
  4. 한국영양식량학회지 v.24 no.4 수종 잎식물 건조물의 급이가 고콜레스테롤 혈증 흰쥐의 혈청지질에 미치는 영향 강정옥;김경국
  5. 한국식품영양과학회지 v.27 no.6 뽕잎 추출물이 콜레스테롤 투여 흰주ㅢ이 혈청지질에 미치는 영향 김선여;이완주;김현복;김애정;김순경
  6. Lipid v.19 Species, tissue and subcellular distribution of non Se-dependent glutathione peroxidase activity Lawrence, R. A.;Burk, R. F.
  7. 韓蠶學誌 v.40 no.1 잠상산물의 장기간 투여에 따른 혈당강화효과 이희삼;정교순;김선여;류강선;이완주
  8. Methods Enzymol. v.1986 Determination of carbonyl content in oxidatively modified proteins Levine, R. L.;Garland, D.;Oliver, C. N.;Amici, A.;Climent, I.;Lenz, A. G.;Ahn, B.;Shaltiel, S.;Stadtman, E. R.
  9. J. Biol. Chem. v.193 Protein measurement with the Folin-Phenol reagent Lowry, O. H.;Roseborough, N. J.;Farr, L. A.;Randall, R. J.
  10. J. B. Chem. v.244 no.22 Superoxide dismutase. An enzymic function for erythrocuprein(hemocuprein) McCord, J. M.;Fridovch, I.
  11. Free Radical Biology & Medicine v.20 no.1 Suppression of inflammatory arthritis by simultaneous inhibition of nitric oxide synthase and NADPH oxidase Miesel, R.;Kurpisz, M.;Kroger, H.
  12. Clin. Chem. v.24 Simultaneous determination of serum cholesterol in high and low density lipoprotein with usse of heparin,$Ca^{2+}$and an anion exchange resin Noma, A.;Nakayama, K. N.;Kita, M.;Okabe, H.
  13. Anal Biochem v.42 Reevaluation of assay methods and establishment of Kit for superoxide dismutase activity Oyanagui, Y.
  14. Agric. Chem. Biotech. v.41 no.2 Cytotoxic activity of Bombyx mori and Morus alba derived materials against human tumor cell lines Park, I. K.;Lee, J. O.;Lee, H. S.;Seol, K. Y.;Ahn, Y. J.
  15. Anal. Biochem. v.81 Simultaneous determination of superoxide dismutase and catalase in biological materials by polarogtaphy Rigo, A.;Rotilio, G.
  16. J. Lipid Res. v.14 Determination of cholesterol using o-phthalaldehyde Rudel, L. L.;Morris, AM. D.
  17. J. Nutr. v.122 no.35 A current perspective on nutrition and excercise Singh, V. N.
  18. Eur. J. Biochem. v.25 Hepatic microsomal ethanol oxidation Thurman, R. G.;Ley, H. G.;Scholz, R.
  19. Chemistry and Physics of Lipids v.45 Lipid peroxides and human diseases Yagi, K.
  20. J. Biol. Chem. v.261 Extraction and identification of antioxidant components from the leaves of mulberry (Morus alba L.). Yen, G. C.;Wu, S. C.;Duh, P. D.
  21. J. Biol. Chem. v.261 Extraction and identification of antioxidant components from the leaves of mulberry (Morus alba L.). Yen, G. C.;Wu, S. C.;Duh, P. D.
  22. Agric Biol. Chem. v.52 Inhibition of intestinal α-glucosidase activity and postprandial hyperglycemia by moranoline and its N-alkyl derivatives Yoshikumi, Y.
  23. Ann. N. Y. Acad. Sci. v.786 Critical evaluation of free radical theory of aging: A proposal of oxidative stress hypothesis Yu, B. P.;Yang, R.
  24. Free Rad. Biol. Med. v.21 Aging and oxidative stress : Modulation by dietary restriction Yu, B. P.
  25. 機能性食品に關する共同社業報告 v.2 官原智江子;佐藤修二;官澤眞紀;堀口佳哉;淸水昭男;原田昌與
  26. 原色和漠藥圖鑑 下券 難波恒雄
  27. 糖尿病動物 v.2 木村勞
  28. 新藥と臨床 v.47 no.2 桑葉による二糖糖類分解酵素の沮害果 飯喙草澄;橈井樂一;强地發;澤田雅彦;石家駿治
  29. 月刊フ-ドケミカル v.12 桑の葉茶の成人病に對する果 野田信三
  30. 神奈川試驗硏究報告書 論文集 鈴本誠
  31. 機能性 食品に關する共同硏究報告 v.2 鈴本誠;高僑一;坂本聖五;有賀動
  32. 農化 v.50 no.11 桑のビペリヅン型マルカロイドの構造について 八木井廣;河野長彦;靑柳食明;村井博