Preventive Nutrition and Food Science
- Volume 4 Issue 3
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- Pages.167-170
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- 1999
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- 2287-1098(pISSN)
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- 2287-8602(eISSN)
Detectio of Malonaldehyde-thiobarbituric Acid (MA-TBA) Complex by High Performance Liquid Chromatography(HPLC) in a Model System
- Whang, Key (Department of Food Science and Technology , Keimyung University)
- Published : 1999.09.01
Abstract
Various concentrations of malonaldehyde (MA) produced upon hydrolysis of 1, 1, 3,3-tetraethoxypropane (TEP) were reacted with 2-thiobarbituric acid (TBA)and th e contents of MA-TBA complex were measured both with spectrophotometer and high performance liquid chromatography (HPLC). As the concentrations of MA-TBA increased, their absorbances and the corresponding HPLC peak areas increased. The correlation coefficient between absorbances and HPLC peak areas of MA-TBA peaks from the other compounds and butanol extraction of the complex increased its recovery its recovery by 29.4% . Measurement of the content of MA-TBA complex for monitoring the development of lipid oxidation was proven to be successful with the use of high performance liquid chromatography.
Keywords
- model system;
- high performance liquid chromatography;
- MA-TBA complex;
- ion pairing agent;
- butanol extraction