Biotechnology and Bioprocess Engineering:BBE

  • 제4권1호
  • /
  • Pages.36-40
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  • 1999
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  • 1226-8372(pISSN)
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  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Production of Lyopilized Culture of Lactobacillus acidophilus with Preserving Cell Viability

  • Kang, Moo-Heon (Bioprocess Eng. Lab., Hanhyo Institute of Technology, Taejon) ;
  • Vibhor Saraswat (Biochemical Process Engineering R. U., Korea Research Institute of Bioscience and Biotechnology, (KRIBB), Taejon) ;
  • Lee, Jeewon (Biochemical Process Engineering R. U., Korea Research Institute of Bioscience and Biotechnology, (KRIBB), Taejon) ;
  • Park, Young-Hoon (Biochemical Process Engineering R. U., Korea Research Institute of Bioscience and Biotechnology, (KRIBB), Taejon)
  • 발행 : 1999.03.01
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초록

Optimal lyophilization process was developed for manufacturing the dried product of Lactobacillus acidophilus with high cell viability. Three major factors, freezing rate, specific surface area of samples, and stabilizer type and their synergy were shown to play a crucial role in the development of an effective lyophilization process. Finally we found an optimal combination among three process parameters mentioned above; an exceptionally high cell survival percentage of 90% was achieved using the 8.28 cm-1 specific surface area of samples, slow freezing rate, and a stabilizer composition of 4% skim milk +1% glycerol +0.1% calcium chloride.

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참고문헌

  1. Microbiologica v.7 Colonization of a human intestine by four different genotypes of Lactobacillus acidophilus Sarra, P. G.;F. Dellaglio
  2. Eur. Patent.0416 892 Onishi, N.;A. Yamashiro
  3. Appl. Environ. Microbiol. v.61 Intestinal flora of populations that have a high risk of colon cancer Moore, W. E. C.;L. H. Moore
  4. J. Med. Chem. v.41 Stucture-based design and synthesis of lipophilic 2,4-diamino-6-substituted quinazolines and their evaluation as inhibitors of dihydrofolate reductases and potential antitumor agents Gangjee, A. A. P.;A. Vidwans;S. F. Vasudevan;R. L. Queener;V. Kisliuk;R. M. Cody;N. Li;J. R. Galitsky;L.S. Pangborn
  5. Cancer Immunol. Immunother. v.42 Suppressive effects of Lactobacillus casel cells, a bacterial immumostimulant, on the incidence of spontaneous thymic lymphoma in AKR mice Watanabe, T.
  6. FEMS Microbiol. Rev. v.16 Technological and economic potential of poly (lactic acid) and lactic acid derivatives Datta, R.;S. P. Tsai;P. Bonsignore;S. H. Moon;J. R. Frank
  7. Appl. Biochem. Biotechnol. v.70;72 An integrated bioconversion process for production of L-lactic acid from starchy potato feedstocks Tsai, S. P.;S. H. Moon
  8. Appl. Biochem. Biotechnol. v.11 Continuous production of lactic acid in a cell recycle reactor Ohleyer, E.;H. W. Balanche;C. R. Wilke
  9. J. Appl. Bact. v.23 A medium for the cultivation of Lactobacillii De Man, J. C.;M. Rogosa;M. E. Sharpe
  10. Biotechnol. Lett. v.20 Continuous cultication of a human intestinal microflora at high cell concentration via controlled culture recycle Kang, M. H.;J. Lee
  11. J. Dairy Sci. v.78 Properties of Lactobacillus heleveticus CNRZ-32 attenuated by spray-drying, freeze-drying, or freezing Johnson, J. A. C;M. R. Etzel
  12. J. Dairy Sci. v.80 Influence of bile on β-galactosidase activity and cell viability of Lactobacillus reuteri when subjected to freeze-drying De valdez, G. F.;G. Martos;P. Taranto;G. L. Lorca;G. Oliver;A.P. de Ruiz Holgado
  13. Appl. Biochem. Biotechnol. v.70;72 Improvement of lactic cell production Desmons, S.;H. Krhous;P. Evrard;P. Thonart
  14. J. Food Sci. v.48 Survival of Lactobacillus bulgaricus during freezing and freeze-drying after growth in the presence of calcium Wrigth, C. T.;T. R. Klaenhammer
  15. Int. J. Pharm. v.62 The cake collapse temperature in freeze drying; dependence on measurement methodology and rate of water removal from the glassy phase Pikal, M. J.;S. Shah
  16. Cryobiology v.20 Viability of micrococci and lactobacilli upon freezing and freeze-drying in the presence of differnet cryoprotectants Tsvetkov, T;R. Brankova
  17. Appl. Environ. Microbiol. v.45 Protective effect of adonitol on lactic acid bacteria subjected to freeze-drying De Valdez, G. F.;G. S. de Giori;A. A. P. de Ruiz Holgado;G. Oliver
  18. Cryobiology v.20 Comparative study of the efficiency of some addives on protecting lactic acid bacteria aginst freeze-drying De Valdez, G. F.;G. S. de Giori;A. A. P. de Ruiz Holgado;G. Oliver
  19. Cryo-Lett. v.10 The rate of glycerol and DMSO on the freezing of spermatozoa Maeda, T.;T. Terada; Y. Tsutsumi