Biotechnology and Bioprocess Engineering:BBE

  • 제4권1호
  • /
  • Pages.32-35
  • /
  • 1999
  • /
  • 1226-8372(pISSN)
  • /
  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Increased Production of Digoxin by Digitoxin Biotransformation Using Cyclodextrin Polymer in Digitalis lanata Cell Cultures

  • Lee, Jong-Eun (Department of Biological Engineering, Inha University, Inchon) ;
  • Lee, Sang-Yoon (Department of Biological Engineering, Inha University, Inchon) ;
  • Kim, Dong-Il (Department of Biological Engineering, Inha University, Inchon)
  • 발행 : 1999.03.01
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초록

Addition of ${\beta}$-cyclodextrin (${\beta}$-CD) polymer during the biotransformation of digitoxin into digoxin using cell suspension cultures of Digitalis lanata enhanced the conversion yield. Digitoxin showed better adsorption to CD polymer compared to digoxin, so that the optimization of addition time was found to be necessary. In the case of adding CD polymer 24 hours after the feeding of substrate digitoxin, the highest digoxin production could be achieved. At this period, digitoxin was almost consumed by cells and productivity was proportionally enhanced according as the amount of substrate was increased. Immobilization of CD polymer did not promote the biotransformation. When 3.33 g/L of CD selective inclusion complex formation could be expected. Adsorption rate was found to be rapid and saturation was obtained within 10 hours of contact.

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