Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Protective Effects of Antoxidant Enzymes of Candida albicans against Oxidative Killing by Macrophages

  • Kim, Hye-Jin (Department of Biology, College of Natural Science Chung-Ang University) ;
  • Na, Byoung-Kuk (Department of Biology, College of Natural Science Chung-Ang University) ;
  • Kim, Moon-Bo (Department of Biology, College of Natural Science Chung-Ang University) ;
  • Park, Duk-Young (Department of Neurosurgery, College of Medicine, Chung-Ang University) ;
  • Song, Chul-Yong (Department of Biology, College of Natural Science Chung-Ang University)
  • Published : 1999.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Protective roles of antioxidant enzymes, copper-zinc superoxide dismutase (CuZnSOD), manganese superoxide dismutase (MnSOD), and catalase of Candida albicans against exogenous reactive oxygens and oxidative killing by macrophages were investigated. The initial growth of C. albicans was inhibited by reactive, oxygen-producing chemicals such as hydrogen peroxide, pyrogallol, and paraquat, but it was restored as the production of antioxidant enzymes were increased. The growth inhibition of C. albicans by reactive, oxygen-producing chemicals was reduced by treating the purified candidal SOD and catalase. Also, in the presence of SOD and catalase, the oxidative killing of C. albicans by macrophages was significantly inhibited. These results suggest that antioxidant enzymes, CuZnSOD, MnSOD, and catalase of C. albicans may play important roles in the protection of C. albicans not only from exogenous oxidative stress but also from oxidative killing by macrophages.

Keywords

References

  1. N. Engl. J. Med. v.298 Oxygen-dependent microbial killing by phagocytes Babior, B.M.
  2. J. Gen. Microbiol. v.131 A comparison of phospholipase activity, cellular adherence and pathogenicity of yeasts Barrett-Bee, K.;Y. Hayes;R.G. Wilson;J.F. Ryley
  3. Ann. Rev. Microbiol. v.38 The role of oxygen and its derivatives in microbial pathogenesis and host defense Beaman, L.;B.L. Beaman
  4. Infect. Immun. v.58 Monoclonal antibodies demonstrate that superoxide dismutase contributes to protection of Nocardia asteroides within the intact host Beaman, L.;B.L. Beaman
  5. Infect. Immun. v.47 Role of superoxide dismutase and catalase in determinants of pathogenicity of Nocardia asteroides: important in resistance to microbicidal activities of human polymorphonuclear neutrophils Beaman, B.L.;C.M. Black;F. Doughty;L. Beaman
  6. Parasitol. Today v.4 Helminth antioxidant enzymes: A protective mechanism against host oxidants? Callahan, H.L.;R.K. Crouch;E.R. James
  7. Curr. Top. Microbiol. Immunol. v.138 Recent developments in the study of virulence of Listeria monocytogenes Chakraborty, T.;W. Goebel
  8. J. Clin. Invest. v.61 Damage to pseudohyphal forms of Candida albicans by neutrophils in the absence of serum in vitro Diamond, R.R.;B. Krzesicki;W. Jao
  9. Fungal infections and immune responses Introduction to Candida. Systemic candidiasis Domer, J.E.;R.I. Lehre;J.W. Murphy(ed.);H. Friedman(ed.);M. Bendinelli(ed.)
  10. Infect. Immun v.65 Role of an aspartic proteases in disseminated Candida albicans infection in mice Fallon, K.;K. Bausch;J. Noonan;E. Huguenel;P. Tamburini
  11. Infect. Immun. v.58 Contribution of superoxide dismutase and catalase activities to Shigella flexneri pathogenesis Franzon, V.L.;J. Arondel;P.J. Sansonetti
  12. Nippon. Ishinkin. Gakkai. Zasshi. v.39 Extracellular phospholipase as universal virulence factor in pathogenic fungi Ghannoum, M.A.
  13. Rev. Infect. Dis. v.11 Fungemia caused by Candida species and Torulopsis glabrata in the hospitalized patient Komshian, S.V.;A.K. Veaydah;J.D. Sobel;L.R. Crane
  14. J. Med. Microbiol. v.47 Evaluation of phospholipase activity of Candida albicans and its correlation with pathogenicity in mice Kothavade, R.J.;M.H. Panthaki
  15. Nature v.227 Cleavage of structural protein during the assembly of the head of bacteriophage T4 Laemmli, D.K.
  16. Inflammation v.21 Macrophage-mediated candidacidal activity is augmented by exposure to eosinophil peroxidase: a paradigm for eosinophil-macrophage interaction Lefkowitz, D.L.;J.A. Lincoin;K.R. Howard;R. Stuart;S.S. Lefkowitz;R.C. Allen
  17. J. Infect. Dis. v.173 Phagocytosis and intracellular killing of Candida albicans by macrophages exposed to myeloperoxidase Lefkowitz, S.S.;M.P. Gelderman;D.L. Lefkowitz;N. Moguilevsky;A. Bollen
  18. Mycopathologia v.113 Phagocytemediated killing of Candida albicans Lindemann, R.A.;C.K. Franker
  19. Microbiol v.140 Pathogenicity determinants of Candida albians: potential targets for jimmunotherapy? Matthews, R.C.
  20. J. Microbiol. v.35 Purification and characterization of extracellular aspartic proteinase of Candida albicans Na, B.K.;S.I. Lee;S.O. Kim;Y.K. Park;G.H. Bai;S.J. Kim;C.Y. Song
  21. J. Med. Microbiol. v.47 Effect of granulocyte-macrophage colonystimulating factor on candidacidal activity of neutrophils, monocytes or monocyte-derived macrophages and synergy with fluconazole Natarajan U.;N. Randhawa;E. Brummer;D.A. Stevens
  22. Nippikaisi v.92 Superoxide dismutase Noritaka, O.;M. Sinbu;T. Masto;O. Akila
  23. Abstract No. S5.5 Role of Candida acid proteinase in adhesion and invasion of murine epidermis Ray, T.L.;C.D. Payne
  24. FEBS Lett. v.41 Microbial assimilation of methanol induction and function of catalase in Candida boinii Roggenkamp, R.;H. Sahm;F. Wagner
  25. J. Bacteriol. v.176 The iron superoxide dismutase of Legionella pneumophila is essential for viability Sadosky, A.B.;J.W. Wilson;H.M. Steinman;H.A. Shuman
  26. J. Exp. Med. v.152 Macrophage microbicidal activity: Correlation between phagocytosis associated oxidative metabolism and the killing of Candida by macrophages Sasada, M.;D.B. Johnston, Jr.
  27. J. Bacteriol. v.177 Function and stationary-phase induction of periplasmic copperzinc superxxide dismutase and catalase/peroxidase in Caulobacter crescentus Schnell, S.;H.M. Steinman
  28. Ann. Rev. Microbiol. v.39 Candida albicans: Biology genetics, and pathogenicity Shepherd, M.G.
  29. Int J Immunopharmacol. v.19 Upregulation of phagocytosis and candidacidal activity of macrophages exposed to the immunostimulant acemanan Stuart, R.W.;D.L. Lefkowitz;J.A. Lincoln;K. Howard;M.P. Gelderman;S.S. Lefkowitz
  30. Infect. Immun. v.60 Construction of Cu-Zn superoxide dismutase deletion mutants of Brucella abortus: analysis of survival in vitro in epithelial and phagocytic cells and in vivo in mice Tatum, F.M.;P.G. Detilleux;J.M. Sacks;S.M. Halling
  31. Microbiol. Mol. Biol. Rev. v.61 Macrophages in resistance to candidiasis Vazquez-Torres, A.;E. Balish
  32. J. Gen. Microbiol. v.133 Susceptibility of microorganisms to active oxygen species: Sensitivity to the xanthine oxidase-mediated antimicrobial system Yamada, Y.;H. Saito;H. Tomioka;J. Jidoi