Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Tumor Necrosis Factor Receptor (TNFR)-associated factor 2 (TRAF2) is not Involved in GM-CSF mRNA Induction and TNF-Mediated Cytotoxicity

  • Kim, Jung-Hyun (Department of Biochemistry and Molecular Biology, Hanyang University) ;
  • Cha, Myung-Hoon (Department of Biochemistry and Molecular Biology, Hanyang University) ;
  • Lee, Tae-Kon (Department of Biochemistry and Molecular Biology, Hanyang University) ;
  • Seung, Hyo-Jun (Department of Biochemistry and Molecular Biology, Hanyang University) ;
  • Park, Choon-Sik (Division of Allergy and Respiratory Medicine, Department of Internal Medicine, Soonchunhyang University Hosptial) ;
  • Chung, Il-Yup (Department of Biochemistry and Molecular Biology, Hanyang University)
  • Published : 1999.06.01
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Abstract

Tumor necrosis factor receptor (TNFR)-associated factor 2 (TRAF2) is known to act as a signal transducer that connects TNFR2 to its downstream effector functions such as proliferation of thymocytes, regulation of gene expression, and cell death. TRAF2 consists of largely two domains, the N-terminal half that contains a signal-emanating region and the C-terminal half that is responsible for binding to the intracellular region of TNFR2. In this study, we examined the possible roles of TRAF2 in granulocyte-macrophage colony-stimulating factor (GM-CSF) gene expression and cell death. A truncated mutant of TRAF2 ( 2-263) that contains only a C-terminal half was generated, and transiently transfected to the A549 cell, a human lung cancer cell line, and L929 cell, a murine TNF-sensitive cell line. GM-CSF mRNA was induced in untransfected A540 cells both in dose- and time-dependent manner upon the exposure of TNF. However, neither the full length TRAF2 nor the mutant altered GM-CSF mRNA production regardless of the presence or absence of TNF. Furthermore, neither TRAF2 versions significantly changed the cytotoxic effect of TNF on L929 cells. These data suggest that TRAF2 may not be involved in the signal transduction pathway for GM-CSF gene induction and cell death mediated by TNF.

Keywords

References

  1. Anal. Biochem. v.162 Single step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction Chomczynski, P.;N. Sacchi
  2. J. Biochem. Mol Biol. v.29 Cell surface expression of tumor necrosis factor-alpha by activated rat astrocytes Chung, I.Y.;E.N. Benveniste
  3. J. Immunol. v.149 Role of protein kinase C activity in tumor necrosis factor-α gene expression: involvement at eht transcriptional level Chung, I.Y.;J. Kwon;E.N. Benveniste
  4. J. Biol. Chem. v.265 Antibodies to a soluble form of a tumor necrosis factor(TNF) receptor have TNF-like activity Engelmann, H.;H. Holtmann;C. Brakebush;Y.S. Avni;I. Sarov;Y. Nophar;E. Hadas;O. Leitner;D. Wallach
  5. J. Exp. Med. v.171 Characterization of binding and biological effects of menoclonal antibodies against a human necrosis factor receptor Espevik, T.;M. Brockhaus;H. Leotscher;U. Nonstad;R. Shalaby
  6. J. Immunol. Methods v.95 A highly sensitive cell line, WEHI164 clone 13, for measuring cytotoxic factor/tumor necrosis factor from human monocytes Espevik, T.;J. Nissen-Meyer
  7. J. Immunol. v.149 Both tumor necrosis factor receptor types mediate proliferative signals in human mononuclear cell activation Gehr, G.;R. Gentz;M. Brockhaus;H. Loetscher;W. Lesslauer
  8. Cell v.70 The p70 tumor necrosis factor receptor mediates cytotoxicity Heller, R.A.;K. Song;N. Fan;D.J. Chang
  9. Cell v.84 TRADD-TRAF2 and TRADD-FADD interactions define two distinct TNF receptor 1 signal transduction pathways Hsu, H.;H. Shu;M. Pan;D.V. Goeddel
  10. Cell v.81 The TNF receptor 1-associated protein TRADD signals cell death and NF-κB activation Hsu, H.;J. Xiong;D.V. Goeddel
  11. Proc. Natl Acad. Sci. USA. v.91 Novel role of the 75-κDa tumor necrosis factor (TNF) receptor: inhibition of early hematopoiesis Jaclbsen, F.W.;M. Rothe;L. Rusten;D.V. Goeddel;E.B. Smeland;O.P. Veiby;L. Slordal;S.E.W. Jacobsen
  12. Nature v.227 Cleavage of structural proteins during the asembly of the head of bacteriophage T4 Laemmli, U.K.
  13. Immunity v.7 TRAF2 is essential for JNK but not NF-kappaB activation and regulates lymphocyte proliferation and survival Lee, S.Y.;A. Reichlin;A. Santana;K.A. Sokol;M.C. Nussenzweig;Y. Choi
  14. Mol. Cell. Biol. v.11 Characterization of the mouse granulocyte-macrophage colony-stimulating factor(GM-CSF) gene promoter: nuclear factors that interact with an element shared by three lymphokine genes-those for GM-CSF, interleukin-4(IL-4), and IL-5 Miyatake, S.;J. Shlomai;K. Arai;N. Arai
  15. Jpn. J. Cancer Res. v.85 Autonomous expression of cytokine genes by human lung cancer cells and their paracrine regulation Mizuno, K.;S. Sone;E. Orino;A. Nii;T. Ogura
  16. Nature v.323 Recombinant human TNF induces production of granulocyte-monocyte colony-stimulating factor Munker, R.;J. Gasson;M. Ogawa;H.P. Koeffler
  17. Cell v.70 Mice deficient for the 55kd tumor necrosis factor receptor are resistant to endotoxic shock, yet succumb to L. monocytogenes infection Pfeffer, K.;T. Matsuyama;T.M. Kundig;A. Wakeham;K. Kishihara;A. Shahinian;K. Wiegmann;P.S. Ohashi;M. Kronke;T.W. Mak
  18. Science v.269 TRAF2-mediated activation of NF-κB by TNF receptor 2 and CD40 Rothe, M.;V. Sarma;V.M. Dixit;D.V. Goeddel
  19. Cell v.78 A novel family of putative signal transducers associated with the cytoplasmic domain of the 75 kDa tumor necrosis factor receptor Rothe, M.;S.C. Wong;W.J. Henzel;D.V. Goeddel
  20. Molecular: A laboratory manual(second edition) Sambrook, J.;E.F. Fritisch;T. Maniatis
  21. Cell v.76 The TNF receptor superfamily of cellular and viral proteins: activation, costimulation, and death Smith, C.A.;T. Farrah;R.G. Goodwin
  22. Proc. Natl. Acad. Sci. USA. v.93 The tumor necrosis factor-inducible zinc finger protein A20 interacts with TRAF1/TRAF2 and inhibits NF-kappaB activation Song, H.Y.;M. Rothe;D.V. Goeddel
  23. Immunol. Today v.13 Two TNF receptors and signal transduction Tartaglia, L.A.;D.V. Goeddel
  24. J. Biol. Chem. v.267 Tumor necrosis factor receptor signaling: a dominant negative mutation suppresses the activation of the 55-kDa tumor necrosis factor receptor Tartaglia, L.A.;D.V. Goeddel
  25. J. Immunol. v.151 Stimulation of human T cell proliferation by specific activation of the 75-kDa tumor necrosis factor receptor Tartaglia, L.A.;D.V. Goeddel;C. Reynolds;I.S. Figari;R.F. Weber;B.M. Fendly;M.A. Palladino
  26. Proc. Natl. Acad. USA. v.88 The two different receptors for tumor necrosis factor mediate distinct cellular responses Tartaglia, L.A.;R.F. Weber;I.S. Figari;C. Reynolds;M.A. Palladino;D.V. Goeddel
  27. Proc. Natl. Acad. Sci. USA v.94 CD30 induction of human immunodeficiency virus gene transcription is mediated by TRAF2 Tsitsikov, E.N.;D.A. Wright;R.S. Geha
  28. J. Exp. Med. v.176 Functional characterization of the human tumor necrosis factor receptor p75 in a transfected rat/mouse T cell hybridoma Vandenabeele, P.;W. Declercq;D. Vercammen;M. Van de Craen;J. Grooten;H. Loetscher;M. Brockhaus;W. Lesslauer;W. Fiers
  29. Science v.228 Human GM-CSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins Wong, G.G.;J.S. Witek;P.A. Temple;K.M. Wilkens;A.C. Leary;D.P. Luxenberg;S.S. Jones;E.L. Brown;R.M. Kay;E.C. Orr(et al.)