Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Purification and Characteristics of Glucoamylase in Aspergillus oryzae NR 3-6 Isolated from Traditional Korean Nuruk

  • Yu, Tae-Shick (Department of Microbiology, Keimyung University, Taegu Korea) ;
  • Kim, Tae-Hyoung (Department of Microbiology, Keimyung University, Taegu Korea) ;
  • Joo, Chong-Yoon (Department of Parasitology, School of Medicine, Keimyung University, Taegu)
  • Published : 1999.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

The purification system of glucoamylase (glucan 1,4-${\alpha}$-glucosidase, EC 3. 2. 1. 3), some characteristics of the purified enzyme and hydrolysis rate of various raw starch were investigated through several experiments. The enzyme was produced on a solid, uncooked wheat bran medium of Aspergillus oryzae NR 3-6 isolated from traditional Korean Nuruk. The enzyme was homogeneously purified 6.8-fold with an overall yield of 28.3% by the criteria of disc- and SDS-polyacrylamide gel electrophoresis. The molecular weight was estimated to be 48 kDa by SDS-PAGE. The optimum temperature and pH were 55$^{\circ}C$ and 4.0, respectively. The enzyme was stable at a pH range of 3.0∼10.0 and below 45$^{\circ}C$. Enzyme activity was inhibited about 27% by 1mM Hg2+. The hydrolysis rate of raw wheat starch was shown to be 17.5-fold faster than the hydrolysis rate of soluble starch. The purified enzyme was identified as glucoamylase because the product of soluble starch by the purified enzyme was mainly glucose by thin layer chromatography.

Keywords

References

  1. Clin. Chem. v.20 Enzymatic determination of total serum cholesteerol Allain, C.C.;L.S. Poon;C.S.G. Chan;W. Richmond;P.C. Fu
  2. Anal. Biochem. v.72 A rapid and sensitive method for the quantitation of protein utilizing the principle of proteindye binding Bradford, M.M.
  3. Kor. J. Appl. Microbiol. Biotechnol. v.25 Purification and characteristics of raw starch hydrolyzing enzyme from Aspergillus niger Chung, M.J.
  4. Kor. J. Appl. Microbiol. Biotechnol. v.18 Studies on the development and the characteristics of the powerful raw starch digesting enzyme Chung, M.J.;W.N. Hou;J.H. Jeong;J. Tanigachi
  5. Ann. New York Acad. Sci. v.121 Disc electrophoresis II. Method and application to human serum protein Davis, B.J.
  6. Agric. Biol. Chem. v.46 Evidence for raw starch-affinity site on Aspergillus awamori glucoamylase I Hayashida, S.;S. Kunisaki;M. Nakao;P.Q. Flor
  7. J. Inst. Nat. Sci. Keimyung University v.15 Geneeral characterization of traditional Korean Nuruk Kim, H.S.;J.S. Hyun;J. Kim;H.P. Ha;T.S. Yu
  8. J. Korean Soc. Food Sci. Nutr. v.26 Characteristics of useful fungi isolated from tarditional Korean Nuruk Kim, H.S.;J.S. Hyun;J. Kim;H.P. Ha;T.S. Yu
  9. Kor. J. Appl. Microbiol. biotechnol. v.26 Enzymological characteristics and identification of useful fungi isolated from traditional Korean Nuruk Kim, H.S.;J.S. Hyun;J. Kim;H.P. Ha;T.S. Yu
  10. Korean J. Food Sci. Technol. v.18 Purification characterization of raw starch digestion enzyme from Rhizopus oryzae Kim, C.J.;M.J. Oh;J.S. Lee
  11. Agric. Biol. Chem. v.52 Effective purification of glucoamylase in koji a solid culture of Aspergillus oryzae on steamed rice, by afinity chromatography using an immobilized acabose (BAY g-5421) Ono, K.;S. Shigeta;S. Oka
  12. J. Ferment. Technol. v.56 Glucoamylase of Aspergillus oryzae Razzaque, A.;S. Ueda
  13. J. Biochem. v.89 Purification and characterization of a glucoamylase from Aspergillus saitoi Takahashi, T.;N. Inokuchi;M. Irie
  14. J. Biol. Chem. v.244 The reliability of molecular weight determination by sodium dodecyl sulfate polyacrylamide gel electrophoresis Weber, K.;M. Osborn
  15. Agric. Biol. Chem. v.41 Purification and properties of two forms of glucoamylase from Penicillium oxalicum Yamasaki, Y.;Y. Suzuki;J. Ozawa
  16. Agric. Bio. Chem. v.41 Properties of two forms glucoamylase from Penicillium oxalicum Yamasaki, Y.;Y. Suzuki;J. Ozawa
  17. Agric. Biol. Chem. v.41 Two forms of glucoamylase from Mucor rouxianus. II. Properties of the two glucoamylase Yamasaki, Y.;A. Tsuboi;Y. Suzuki
  18. Agric. Biol. Chem. v.53 Purification and proterties of two forms of glucoamylase from Monascus sp. No. 3403 Yasuda, M.;M. Kuwae;J. Matsushita
  19. J. Inst. Nat. Sci. Keimyung University v.17 Optimum culture conditions for production of glucoamylase by Aspergillus oryzae NR 3-6 isolated from traditional Korean Nuruk Yu, T.S.;T.H. Kim
  20. J. Korean Soc. Food. Nutr. v.25 Bibligraphical study on microorganisms of Nuruk (until 1945) Yu, T.S.;H.S. Kim;J. Hong;J.P. Ha;T.Y. Kim;I.W. Yoon