Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Degradation of Anthracene by a Pseudomonas strain, NGK1

  • Shinde Manohar (Department of Biochemistry, Gulbarga University, India) ;
  • Kim, Chi-Kyung (Department of Microbiology, Chungbuk National University, Cheongju) ;
  • Tim (Department of Biochemistry, Gulbarga University, India)
  • Published : 1999.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Pseudomonas sp. NGK1, isolated by naphthalene enrichment culture technique, is capable of degrading anthracene as a sole source of carbon and energy. The organism degraded anthracene through the intermediate formation of 1,2-dihydroxyanthracene, 2-hydroxy-3-naphthoic acid, salicylate, and catechol. The intermediates were isolated and characterized by TLC, spectrophotometry, and HPLC analysis. The cell free extract of anthracene-grown cells showed activities of anthracene dioxygenase, 2-hydroxy-3-naphthylaldehyde dehydrogenae, 2-hydroxy-3-naphthoate hydroxylase, salicylate hydroxylase and catechol 2,3-dioxygenase. The formed catechol as a metabolite is degraded through meta-cleavage with the formation of ${\alpha}$-hydroxymuconic semi-aldehyde.

Keywords

References

  1. BioSci. v.45 Bioremediation of petroleum pollutants Atlas, R.M.;C.E. Cerniglia
  2. J. Bacteriol. v.153 Bacterial degradation of naphthalene and phenanthrene Barnsley, E.A.
  3. Adv. Appl. Microbiol. v.30 Microbial degradation of polycyclic aromatic hydrocarbons Cerniglia, C.E.
  4. Ann Microbiol. Enzymol. v.9 Ricereche sul metabolismo ossidativo microbiwdell anthracene e dalfen antrene Cola, A.;A. Fiecchi;V. Treccahi
  5. J. Bacteriol. v.174 Bacterial metabolism of naphthalene: construction and use of recombinant bacteria to study ring cleavage of 1,2-dihydroxynaphthalene and subsequent reactions Eaton, R.W.;P.J. Chapman
  6. J. Bacteriol. v.151 Metabolism of dibuthylphthalate and phthalate by Micrococcus sp. strain 12 B Eaton, R.W.;D.W. Ribbons
  7. Biochem. J. v.95 Oxidative metabolism of phenenthrene and anthracene by soil Pseudomonads Evans, W.C.;H.N. Fernly;E. Griffith
  8. Microbial degradation of organic compounds Microbial degradation of aromatic hydrocarbons Gibson, D.T.;V. Subramanian;D.T. Gibson(ed.)
  9. Environmental carcinogenesis of polycyclic aromatic hydrocarbons Grimmer, G.;F. Pott;G. Grimmer(ed.)
  10. Appl. Environ. Microbiol. v.58 Naphthalene degradation via salicylate and gentisate by Rhodococcus sp. strain B4 Grund, E.;B. Denecke;R. Eichenlaub
  11. Oil Sci. v.221 The fate of Amoco cadiz Gundlach, E.R.;P.D. Boehm;M. Marchand;R.M. Atlas;D.M. Ward;D.A. Wolfe
  12. J. Bacteriol. v.91 Synthesis of enzymes of the mandalate pathway by Pseudomonas putida. I Synthesis of enzymes of wild type Hegeman, G.D.
  13. Appl. Environ. Microbiol. v.54 Mineralization of polycyclic aromatic hydrocarbons by a bacterium isolated from sediment below in an oil field Heitkamp, M.A.;C.E. Cerniglia
  14. Arch. Biochem. Bophys. v.142 cis, -1,2-Dihydroxy-1,2-dihydronaphthalene: a bacterial metabolite from naphthalene Jarina, D.M.;J.W. Daly;A.M. Jeffery;D.T. Gibson
  15. J. Am. Chem. Soc. v.98 Dihydrodiol from anthracene and phenanthrene Jarina, D.M.;H. Sclander;H. Yagi;M.C. Wells;J.F. Davey;V. Mahadevan;D.T. Gibson
  16. Environ. Sci. Technol. v.13 Priority Pollutants. 1. A perspective view Keith, L, H,;W,A, Telliard
  17. J. Bacteriol. v.173 Purification and characterization of a 1,2-dihydroxynaphthalene dixygenase from a bacterium that degrades naphthalene sulfonic acids Kuhm, A.E.;A. Stolz;K.L. Ngai;H.J. Knackmuss
  18. J. Bio. Chem. v.193 Protein measurement with folin phenol reagent Lowry, O.H.;N.J. Rosebrough;A.L. Farr;R.J. Randall
  19. Indian J. Expt. Biol. v.33 Degradation of naphthalene by a Pseudomonas strain NGK1 Manohar, S,;T.B. Karegoudar
  20. Prod. Acad. Environ. Biol. v.55 Effect of nitrogen source on the bacterial degradation of naphthalene Manohar, S,;T.B, Karegoudar
  21. Appl. Microbiol. Biotechnol. v.49 Degradation of naphthalene by cells of Pseudomonas sp. strain NGK1 immobilized in alginate, agar, and polyacrylamide Manohar, S,;T.B. Karegoudar
  22. Appl. Environ. Microbiol. v.59 NAH plasmid-mediated catabolism of anthracene and phenanthrene to naphthoic acids Menn, F.M.;B.H. Applegate;G.S. Sayler
  23. Biochem. Biophys. Acta. v.220 Meta-pyrocatechase 111. Substrate specificity and mode of ring fission Nozaki, M.S.;K. Oni;Kotani;S. Senoh
  24. Biodegradation of hazardous organic pollutants Patterson, J.W.;Kodukala
  25. Appl. Environ. Microbiol. v.59 Plasmid mediated mineralization of naphthalene, phenanthrene and anthracene Sanseverion, J.;B.N. Applegate;J.M.H. King;G.S. Sayler
  26. Environment and Lung 'disease Polycyclic aromatic hydrocarbons biodegradation and fate in aquatic sediments Sayler, G.S.;T.W. Sherrill;G. Babolini(ed.);G.Givlbino(ed.);La Grafica(ed.)
  27. J. Gen. Microbiol. v.83 The regulation of naphthalene oxygenase in Pseudomonas Shamsuzzamann K.M.;E.A. Barnsley
  28. J. Biol. Chem. v.247 Studies of a flavoprotein, salicylate hydroxylase Enzyme mechanism White-Stevens, R.H.;Kamin