약학회지 (YAKHAK HOEJI)

  • 제43권3호
  • /
  • Pages.300-305
  • /
  • 1999
  • /
  • 0377-9556(pISSN)
  • /
  • 2383-9457(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

HPLC-ECD를 이용한 흰쥐의 뇌 중 감마 아미노부티르산 및 글루탐산의 정량

Determination of ${\gamma}-Aminobutyric$ Acid and Glutamic Acid in Rat Brain by High Performance Liquid Chromatography with Electrochemical Detection

  • 발행 : 1999.06.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

A sensitive and efficient assay method was applied to determine the level of glutamic acid (GA) and ${\gamma}-aminobutyric$ acid (GABA) in frontal cortex and hippocampus of rat administrated with ethanol and drugs. The compounds were derivatized with ο-phthalaldehyde (OPA) and 2-mercaptoethanof for precolunm analysis. The condition for the simultaneous determination of GA, GABA and $\beta-aminobutyric$ acid (BABA) by high performance liquid chromatography with electrochemical detection was reverse phase $C_{18}$ column as stationary phase, 0.1 M phosphate buffer containing 0.1 mM $Na_4EDTA$ : methanol = 55:45 (v+v) pH 3.8 as mobile phase and 0.7V electrode voltage. The stability of reaction product of GA, GABA and BABA with OPA could be increased by adding the same volume of polyethylene glycol 400 to reaction mixture. The GABA level in frotal cortex of rat was significantly decreased by the administration of picrotoxin and diazepam, but it was significantly increased by the administration of red ginseng total saponin, N-methyl-D-glucamine and (-)-deprenyl.

키워드

참고문헌

  1. Trends in Pharmacol. Sci. v.13 Neurobiology of alcohol abuse Samson,H.H.;Harris,R.A.
  2. Science v.235 Alcoholian the medical cost crunch Holden,C.
  3. Psychopharmacol. v.78 State-dependent effects of ethanol on active and passive avoidance learning Holloway,F.A.
  4. Neuropharmacol. v.22 Benzodiazepine-GABA-receptor-ionophore complex Ticku,M.K.
  5. Eur. J. Pharmacol. v.89 Behavioral evidence for the involvement of gamma-aminobutyric acid in the action of ethanol Martz,A.;Deitrich,R.A.;Harris,R.A.
  6. J. Chromatogr. v.708 Determination of γ-aminobutyric acid in microdialysis samples by microbore column liquid chromatography and fluorescence detection Kehr,J.
  7. J. Chromatogr. v.708 Determination of glutamate and aspartate in microdialysis samples by reversed-phase column liquid chromatography with fluorescence and electrochemical detection Kehr,J.
  8. Neurochem. Res. v.20 Amino acid concentrations and selected enzyme activities in rat auditory, olfactory, and visual systems Ross,C.D.;Godfrey,D.A.;Parli,J.A.
  9. J. Chromatogr. v.579 γ-aminobutyric acid, catecholamine and indoleamine determinations from the same brain region by high-performance liquid chromatography with electrochemical detection Chanpney,T.H.;Hanneman,W.H.;Nichols,M.A.
  10. J. Chromatogr. v.497 Ultra-rapid assay of brain γ-aminobutyric acid by liquid chromatography with electrochemical detection Murai,S.;Saito,H.;Nagahama,H.;Miyate,H.;Masuda,Y.;Itoh,T.
  11. J. Chromatogr. v.662 Rapid assay for γ-aminobutyric acid in mouse brain synaptosomes using gas chromatography-mass spectrometry Palaty,J.;Burton,R.;Abbott,F.S.
  12. J. Chromatogr. v.337 Rapid gas chromatographic-mass spectrometric quantitation of gamma-aminobutyric acid in biological specimens Morman,E.J.;Wee,E.L.;Zimmerman,E.F.
  13. J. Chromatogr. v.694 Improved method for the measurement of glutamate and aspartate using capillary electrophoresis with laser induced fluorescence detection and its application to brain microdialysis Dawson,L.A.;Stow,J.M.;Palmer,A.M.
  14. Anal. Chem. v.68 Quantitative in vivo monitoring of primary amines in rat caudate nucleus using microdialysis coupled by a flow-gated interface to capillary electrophoresis with laser-induced fluorescence detection Lada,M.W.;Kennedy,R.T.
  15. J. Chromatogr. v.700 Capillary electrophoresis of glutamate and aspartate in rat brain dialysate-Improvements in detection and analysis time using cyclodextrins Church,W.H.;Lee,C.S.;Dranchak,K.M.
  16. Anal. Biochem. v.156 Stability of ο-phthalaldehyde-derived isoindoles Jacobs,W.A.;Leburg,M.W.;Madaj,E.J.
  17. Anal. Biochem. v.250 Ethanethiol: a thiol conveying improved properties to the fluorescent product of ο-phthaldehyde and thiols with amines Simons,S.S.Jr.;Johnson,D.F.
  18. J. Chromatogr. v.392 O-phthalaldehyde-sulfite derivatization of primary amines for liquid chromatography-electrochemistry Jacobs,W.A.
  19. J. Chromatogr. v.652 Measurement of GABA in rat brain microdialysates using o-phthaldialdehyde-sulphite derivatization and high-performance liquid chromatography with electrochemical detection Smith,S.;Sharp,T.
  20. Maps and guide to microdisection of the rat brain Palkovits,M.;Brownstein,M.
  21. J. Pharm. Sci. v.11 The functional role of limbic system on memory Lee,S.C.;You,K.H.;Kim,E.B.