BMB Reports
- 제32권4호
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- Pages.405-408
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- 1999
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- 1976-670X(eISSN)
Glutamic Acid Rich Helix II Domain of the HIV-1 Vpu has Transactivation Potential in Yeast
- Hong, Seung-Keun (Division of Life Science, Pai Chai University) ;
- Bae, Yong-Soo (Department of Microbiology, Hannam University) ;
- Kim, Jung-Woo (Division of Life Science, Pai Chai University)
- 발행 : 1999.07.31
초록
The transactivation potential of HIV-1 Vpu was identified from the yeast two-hybrid screening process. The helix II domain of HIV-1 Vpu protein and mutant Vpu protein lacking the transmembrane domain exhibited transactivation of the LacZ and Leu2 reporter genes carrying LexA upstream activating sequences, but full-length HIV-1 Vpu and the helix I domain of HIV-1 Vpu did not. The helix II domain of HIV-1 Vpu consists of a number of acidic amino acids, and is especially rich in glutamic acid, a characteristic of many transcription factors. This result suggests that protein-protein interaction may occur through the acidic helix II domain of HIV-1 Vpu.