The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Effects of Chiyangtang on Helicobacter pylori-induced increase of cytokines gene expression

Helicobacter pylori 감염에 의한 Cytokines 유전자 발현에 대한 치양탕(治瘍湯)의 효과

  • Lee, Hyung-Ju (Department of Gastrointestenal internal medicine, College of oriental medicine, Wonkwang University) ;
  • Won, Jin-Hee (Department of Gastrointestenal internal medicine, College of oriental medicine, Wonkwang University) ;
  • Moon, Goo (Department of Gastrointestenal internal medicine, College of oriental medicine, Wonkwang University) ;
  • Moon, Seok-Jae (Department of Gastrointestenal internal medicine, College of oriental medicine, Wonkwang University) ;
  • Park, Dong-Won (Department of Gastrointestenal internal medicine, College of oriental medicine, Kyunghee University)
  • 이형주 (원광대학교 한의과대학 비계내과학교실) ;
  • 원진희 (원광대학교 한의과대학 비계내과학교실) ;
  • 문구 (원광대학교 한의과대학 비계내과학교실) ;
  • 문석재 (원광대학교 한의과대학 비계내과학교실) ;
  • 박동원 (경희대학교 한의과대학 비계내과학교실)
  • Published : 1999.09.29
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Abstract

Effects of Chiyangtang(CYT) on H. pylori-induced increase of interleukin 8 and interleukin 1 gene expression was studied in Kato Ⅲ cell line, a human stomach epithelial cell line. Treatment of H. pylori to the cell culture signifant!y increased IL-8 and IL-1 mRNA synthesis. When CYT was added along with H. pylori, the increase of IL-8 and IL-1 mRNA synthesis was blocked. Activation of transcription factor $NF-{\kappa}B$ and AP-1 which were known to important in IL-8 and IL-1 gene expression was also studied using chloramphenicol acetyltransferase(CAT) assay. Treatment of H. pylori increased activation of $NF-{\kappa}B$ and AP-l and CYT effectively protected the activation. Electrophoretic mobility shift assay suggested that CYT effectively inhibited DNA binding of $NF-{\kappa}B$ and AP-l to their cognate site. These results suggested that CYT could prevent stomach diseases through the down regulation of IL -8 and IL-l gene expression which might be mediated by the inhibition of $NF-{\kappa}B$ and AP-1 activities and their binding to DNA.

Keywords

References

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