The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Effects of Semen jugrandis on the iNOS Expression and Superoxide Formation in the RAW264.7 Cells

호도(胡挑) 추출물이 마우스 대식세포주인 RAW264.7 세포주의 iNOS 발현 및 Superoxide 형성에 미치는 영향

  • Moon, Goo (Dept. of Internal Medicine, College of Oriental Medicine University) ;
  • Ko, Su-Mi (Dept. of Internal Medicine, College of Oriental Medicine University)
  • 문구 (원광대학교 한의과대학 비계내과학교실) ;
  • 고수미 (원광대학교 한의과대학 비계내과학교실)
  • Published : 1999.03.30
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Abstract

Nitric oxide(NO) is synthesized via the oxidation of L-arginine by a family of nitric oxide synthases(NOS), which are either constitutive(cNOS) or inducible(iNOS). The induction of iNOS in tissues can lead to the sustained production of high concentrations of NO which may exert pro-inflammatory effects including vasodilation. edema, cyototoxicity, and its activity can be mediated by various pro-inflammatory cytokine, including interferon ${\gamma}(INF-{\gamma})$. tumor necrosis factor, IL- 1 and IL-6. The enzyme, iNOS, became a new target for pharmacologcal research with the aim to find new substances for the treatment of chronic inflammatory disorders. Murine macrophages produce large amounts of NO when activated with $TFN-{\gamma}$ plus LPS. The murine macrophage-like cell line, RAW 264.7, is a suitable cell model on which to perform vitro studies regarding the iNOS system. Semen jugrandis is a fatty walnut seed found in Korea. The walnut have been used in foik medicine to improve virility, to relieved asthma, and to relieve constipation. Sesquiterpenelactones were isolated from this plant. In the course of screening for NO inhibitory activity from medicnial plants, the aqueous extract of this plant was found to have a significant activity. The result are summarized as followings. 1. The viability of cells incubated in the presence of semen jugrandis increased mare than non incubated cells. 2. Semen jugrandis suppressed the production of NO in tissues dependent on density. 3. Semen jugrandis suppressed the induction of iNOS in tissues dependent on density can lead to reduced production of NO. 4. Semen jugrandis suppressed the production of superoxide in tissue depend on density. According to the above mentioned results, semen jugrandis could be applied production of NO and superoxide can lead to reduction of chronic inflammatary. And as a depence matter come into a virus of microbe and tumor cells.

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References

  1. 臨床本草學 辛民敎
  2. 申氏本草學 申佶求
  3. 新編中藥大辭典
  4. 中醫學 問答 v.上;下 楊醫普
  5. 종양학 서울대학교 의과대학
  6. 黃帝內經(素問) 王氷
  7. 中醫雜誌 v.32 no.1 中醫'正氣'槪念探析 張慶營
  8. 脾胃理論與臨床 徐復霖
  9. 臨床的中藥治療 楊寶仁
  10. 臟腑辨證論治
  11. 최신 면역학 李淵臺
  12. 병원미생물학 이종훈
  13. J. immunol v.148 Macrophage cytotoxicity against Entomoeba histolyca trophozoites is mediated by nitric oxide from L-arginine. Lin, J.Y.;Chadee, K.
  14. Phamacol. Rev. v.43 Nitric oxide: Physiology, pathophysiology and pharmacology. Moncada, S.;Palmer, R.M.J.;Higgs, E.A.
  15. Cell v.70 Nitric oxide, a novel biological messenger. Lowenstein, C.J.;Snyder, S.H.
  16. Trends Biochem. Sci. v.17 Nitric oxide as a signal in blood vessels. Knowles, R.G.;Moncada, S.
  17. J. Biol. Chem. v.269 Regulation of the biosynthesis of nitric oxide. Nathan, C.;Xie, Q.-W.
  18. J. Biol. Chem v.268 Nitric oxide synthase structure and mechanism. Marletta, M.A.
  19. Curr. Opoin. Immunol. v.3 Role of nitric oxide synthesis in macrophage antimicrobial activity. Nathan, C.F.;Hibbs, J. Jr.
  20. Science v.261 Inhibition of viral replication by interferon-gamma-induced nitric oxide synthase. Karupiah, G.;Xie, Q.W.;Buller, R.M.;Nathan, C.;Duarte, C.;MacMicking, J.D.
  21. Blood v.80 Modulation of granulocytes survival and programmed cell death by cytokines and bacterial products. Collotta, F.;Re, F.;Pollantaruti, N.;Sozani, S.;Mantovani, A.
  22. Blood v.81 Differential induction of apoptosis in undifferentiated and differentiated HL-60 cells by DNA topoisomerase I and II inhibitors. Solary, E.;Bertrand, R.;Khon, K.W.;Pommier, Y.
  23. Clin Exp Immunol v.79 HL-60 cells induced to differentiate toward neutrophils subsequently die via apoptosis. Martin, S.J.;Bradly, J.G.;Cotter, T.G.
  24. Blood v.70 The HL-60 promyelocytic leukaemia cell line: Proliferation, differentiation and cellular oncogene expression. Collins, S.J.
  25. J. Immunol v.149 Activated microglia mediate neuronal cell injury via a nitric oxide mechanism. Chao, C.C.;Hu, S.;Molitor, T.W.;Shasken, E.G.;Peterson, P.K.
  26. Br. J. Pharmacol v.117 Vasodilator response of rat isolated tail artery enhanced by oxygen-dependent, photochemical release of nitric oxide from iron-sulphur-nitrosyls. Flitney, F.W.;Megson, I.L.;Thomson, L, M.;Kennovin, G.D.;Butler, A.R.
  27. J. Immunol v.152 Taxol provides a second signal for murine macrophage tumoricidal activity. Manthey, C.L.;Perera, P.Y.;Salkowski, C.A.;Volgel. S.N.
  28. J. Neurosci. v.9 Northoff. Lipopolysaccharide-free conditions in primary astrocyte cultures allow growth and isolation of microglial cells. Gebicke-Haerter, P.J.;J. Bauer, A.;Schober, H.
  29. J. Immunol. v.145 Leishmania major amastigotes initiate the L-arginine-dependent killing mechanism in IFN-r stimulated macrophages by induction of tumor necrosis factor-${\alpha}$. Green, S.J.;Crawford, R.M.;Hockmeyer, J.T.;Meltzer, M.S.;Nacy, C.A.
  30. 대한의학협회지 v.36 no.3 한국인의 사망원인. 안돈희
  31. 대한면역학회지 v.8 no.1 암면역학과 면역요법 김진복
  32. 대한의학협회지 v.23 no.9 면역요법의 최근동향 김동집
  33. 東醫病理學 文濬典;安圭錫;崔昇勳
  34. 대한의학협회지 v.24 no.6 韓國正常人 末梢血液淋巴球의 Natural Killer(NK) 活動値에 關한 硏究 夏潤文
  35. 中醫雜誌 v.32 no.5 胡桃仁治何種耳, 腦作鳴 竇國祥
  36. Scientific American Review Biological roles of nitric oxide. Snyder, S.H.;Bredt, D.S.
  37. Call. Immunol v.176 Jun, C.D.;Park, S.J.;Choi, B.M.;Kwak, H.J.;Park, Y.C.;Kim, M.S.;Chung, H.T.
  38. Biochem. Biophys. Res. Commun v.230 Reactive oxygen species participate in peroxynitrite induced apoptosis in HL-60 cells. Lin, K.T.;Xue, J.Y.;Sun, F.F.;Wong, P.Y-K.
  39. J. Immunol v.150 Cytokine production by human fetal microglia and astrocytes. Differential induction by lipopolysaccharide and IL-1${\beta}$. Lee, S.C.;Liu, W.;Dickson, D.W.;Brosnan, C.F.;Berman, J.W.