The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
권호 표지

Effects of Sophorae Radix Water Extract on Cultured Rat Myocardial Cells

고삼(苦蔘) 전탕액(煎湯液)이 배양심근세포(培養心筋細胞)에 미치는 영향(影響)

  • Kim, Hyun-Kyu (Dept. of physiology, college of oriental medicine, Wonkwang Univ.) ;
  • Park, Jun-Su (Dept. of physiology, college of oriental medicine, Wonkwang Univ.) ;
  • Kwon, Kang-Beom (Dept. of physiology, college of oriental medicine, Wonkwang Univ.) ;
  • Lee, Ho-Sub (Dept. of physiology, college of oriental medicine, Wonkwang Univ.) ;
  • Han, Jong-Hyun (Dept. of phamacology, college of oriental medicine, Wonkwang Univ.) ;
  • Park, Seung-Taeck (Dept. of Anatomy, college of medicine, Wonkwang Univ.) ;
  • Ryu, Do-Gon (Dept. of physiology, college of oriental medicine, Wonkwang Univ.)
  • 김현규 (원광대학교 한의과 대학 생리학 교실) ;
  • 박준수 (원광대학교 한의과 대학 생리학 교실) ;
  • 권강범 (원광대학교 한의과 대학 생리학 교실) ;
  • 이호섭 (원광대학교 한의과 대학 생리학 교실) ;
  • 한종현 (원광대학교 한의과 대학 약리학 교실) ;
  • 박승택 (원광대학교 의과대학 해부학교실) ;
  • 류도곤 (원광대학교 한의과 대학 생리학 교실)
  • Published : 1999.03.30
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Abstract

In order to elucidate toxic the mechanism of myocardial damage and the protective effect of herbal extract, Sophorae Radix(SR) against myocardiotoxicity, the cytotoxic effect of adriamycin and cardioprotective effect of SR were examined by MTT assay, LDH activity, heart beat rate and light microscopy after cultured myocardial cells derived from neonatal mouse were treated with various concentrations of adriamycin, an inducer of myocardiotoxicity. Adriamycin induced a decrease of cell viability, an increase in the amount of lactate dehydrogenase(LDH), and a decrease in the heart beat rate and a decrease in the number of cells, when administered to cultures myocardial cells in a dose-dependent manner. In cardioprotective effect of SR. SR showed the decrease of amount of LDH, and an increase of heart beating rate and cells in number on cultured myocardial cells damaged by adriamycin. From the above results, it is suggested that adriamycin shows toxic effect in cultured myocardial cells derived from a neonatal mouse, and herbal extract such as SR is very effective in the prevention of adriamycin-induced cardiotoxicity.

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References

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