생약학회지 (Korean Journal of Pharmacognosy)
- 제30권3호
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- Pages.306-313
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- 1999
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- 0253-3073(pISSN)
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- 2288-9299(eISSN)
은행잎에서 분리한 Polyphenol Oxidase의 정제 및 특성
Purificaton and Some Properties of Polyphenol Oxidase from Ginko biloba Leaves
- Seol, Ji-Yeon (College of Pharmacy, Sookmyung Women's University) ;
- Park, Soo-Sun (College of Pharmacy, Sookmyung Women's University) ;
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Kim, An-Keun
(College of Pharmacy, Sookmyung Women's University)
- 발행 : 1999.09.30
초록
Polyphenol Oxidase(PPO) was purified from an extract of Ginkgo biloba leaves by ammonium sulfate fractionation followed by sephadex G-150 column chromatography, which resulted in a 18-fold increase in specific activity. The enzyme was most active at pH 8.5 and the temperature optimum for the PPO catechol oxidation reaction was