Journal of the Korean Chemical Society (대한화학회지)

Korean Chemical Society (대한화학회)
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Antitumor Evaluation of Epigallocatechin Gallate by Colorimetric Methods

비색분석법에 의한 Epigallocatechin Gallate의 항암효과평가

  • Baek, Soon Ok (Chemical Analysis Center, Korean Ginseng & Tobacco Research Institute) ;
  • Kim, Il Kwang (Department of Chemistry, Wonkwang University) ;
  • Baek, Seung Hwa (Department of Chemistry, Wonkwang University) ;
  • Han, Du Seok (Department of Oral Anatomy, Wonkwang University)
  • 백순옥 (한국인삼연초연구소 분석센터) ;
  • 김일광 (원광대학교 자연과학대학 화학과) ;
  • 백승화 (원광대학교 자연과학대학 화학과) ;
  • 한두석 (원광대학교 치과대학 구강해부학교실)
  • Published : 19980800
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Abstract

In the present study, we were evaluated cytotoxic effects of epigallocatechin gallate in human skin melanoma cells such as HTB-69. The light microscopic study showed morphological changes of the treated cells. Disruptions in cell organelles were determined by colorimetric methods; 3-(4,5-dimethyl thiazol-2-yl)-2,5diphenyl-2H-tetrazolium bromide (MTT) assay, neutral red (NR) assay and sulforhodamine B protein (SRB) assay. These results suggest that epigallocatechin gallate retains a potential antitumor activity.

인체 피부흑색종 세포에 대한 epigallocatechin gallate의 세포독성작용을 평가하고, 광학현미경적 관찰을 실시하여 인체 피부흑색종 세포의 형태학적인 변화를 볼 수 있었다. 세포독성은 비색정량분석법, NR정량분석법과 SRB정량분석법으로 측정하였다. 이런 결과는 epigallocatechin gallate에 항암활성을 보여준다.

Keywords

References

  1. Chem. Pharm. Bull. v.18 Yosioka, L.;Nishimura, T.;Matsude, A.;Kitagawa, L.
  2. Chem. Pharm. Bull. v.19 Yosioka, L.;Nishimura, T.;Matsude, A.;Kitagawa, L.
  3. Korean Agri. Chem. Soc. v.35 나효환;백순옥;한상빈;복진영
  4. Cancer Lett. v.96 Liao, S.;Umekita, Y.;Guo, J.;Kokontis, J. M.;Hiipakka, R. A.
  5. Cancer Res. v.55 Yamane, T.;Takahashi, T.;Kuwata, K.;Oya, K.;Inagake, M.;Kitao, Y.;Suganuma, M.;Fujiki, H.
  6. J. Cell. Biochem. Suppl. v.22 Stoner, G. D.;Mukhtar, H.
  7. Biochem. Biophys. Res. Commun. v.214 Liao, S.;Hiipakka, R. A.
  8. J. Immunol. Methods. v.65 Mosmann, T.
  9. J. Tissue Culture Meth. v.9 Borenfreund, E.;Puemer, J. A.
  10. Natl. Cancer Inst. v.82 Skehan, P.;Storeng, S.;Studiero, D.;Monke, A.;McMahon, T.;Vistica, D.;Warren, J. T.;Bodesh, H.;Kenny, S.;Boyd, M. R.