Journal of Microbiology and Biotechnology
- Volume 8 Issue 1
- /
- Pages.92-95
- /
- 1998
- /
- 1017-7825(pISSN)
- /
- 1738-8872(eISSN)
Overexpression of Escherichia coli Thiol Peroxidase in the Periplasmic Space
- Kim, Sung-Jin (Departments of Genetic Engineering, Pai Chai University) ;
-
Cha, Mee-Kyung
(Departments of Biochemistry, Pai Chai University)
;
-
Kim, Il-Han
(Departments of Biochemistry, Pai Chai University)
;
-
Kim, Ha-Kun
(Departments of Genetic Engineering, Pai Chai University)
- Published : 1998.02.01
Abstract
Overproduction of Escherichia coli thiol peroxidase in the periplasmic space was achieved by locating the appropriate gene on a downstream region of the strong T7 promoter. E. coli strain BL21 carrying the recombinant plasmid pSK-TPX was induced by IPTG, lysed, and analyzed by SDS-polyacrylamide gel electrophoresis. A large amount of the overexpressed thiol peroxidase was located in the periplasmic space. A homogeneous thiol peroxidase was obtained from E. coli osmotic shock fluid by simple one-step gel permeation chromatography.
Keywords
- Thiol peroxidase;
- overexpression;
- periplasm;
- T7 promoter;
- E. coli BL21;
- induction;
- gel permeation chromatography