식물조직배양학회지 (Korean Journal of Plant Tissue Culture)

한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지

미치광이풀 모상근 배양에서 적정 sucrose 농도 및 hydroxyapatite 첨가에 의한 tropane alkaloid 생산성 향상

Improvement of Tropane Alkaloid Productivity by Optimization of Sucrose Concentration and Addition of Hydroxyapatite in Hairy root Cultures of Scopolia parviflora

  • 안준철 (서남대학교 생물학과) ;
  • 양선주 (전남대학교 의과대학 내과교실) ;
  • 표병식 (동신대학교 식품생물공학과) ;
  • 최지원 (전남대학교 생물학과 및 호르몬 연구센터) ;
  • 황백 (전남대학교 생물학과 및 호르몬 연구센터)
  • An, Jun-Chul (Department of Biology, Seonam University) ;
  • Yang, Sun-Ju (Department of Internal Medicine, Medicinal College, Chonnam National University) ;
  • Pyo, Byung-Sik (Department of Food and Biotechnology, College of Engineering Dongsin University) ;
  • Choi, Ji-Won (Department of Biology and Hormone Research Center, Chonnam National University) ;
  • Hwang, Baik (Department of Biology and Hormone Research Center, Chonnam National University)
  • 발행 : 1998.01.01
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초록

미치광이풀(Socopolia parviflora Nakai) 모상근 배양에서 모상근 생장과 tropane alkaloid 생산에 대한 sucrose농도와 일부 흡착제의 효과를 조사하였다. 모상근 클론 SP 11은 5% sucrose 농도를 함유한 1/2 B5 배지에서 최적인 생장과 tropane alkaloid 생산을 보였다. 모상근에서 tropane alkaloids 생성양상은 모식물에서와 약간의 차이를 보였고 그 중 식물체에서 검출되지 않는 littorine의 생성이 모상근에서 두드러졌다. 조사된 흡착제(0.01% 1%) 중에서, charcoal 0.01%와 XAD-II l%에서 약간의 생장촉진효과를 보인 것을 제외하고는 그 외 농도나 흡착제(amberlita chitosan)에서는 억제효과 또는 유의적인 변화를 보이지 않았다. 반면에 hydroxyapatite는 미치광이풀 모상근의 생장과 tropane alkaloid 생산에 있어 촉진효과가 있음이 확인되었다.

The effects of sucrose concentration and some absorbents on growth and tropane alkaloid production in hairy root cultures of Scopolia parviflora were investigated. The maximum effect on growth and tropane alkaloid production in hairy root clone SP11 was obtained for 1/2 B5 medium containing 5% sucrose. The production pattern of tropane alkaloid in hairy roots was some different from that of rhizome of mother plant, particulary showing high littorine contents, which was not found in ordinary roots. Among absorbents examined, charcoal 0.01% and XAD-II 1% made a slight growth promotion effect, whereas the other concentration of charcoal, XAD-II and absorbents (amberlite and chitosan) showed inhibition or no significant effect. The addition of hydroxyapatite enhanced the production of tropane alkaloids significantly than control cultures.

키워드

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